江苏省1例HIV长期无进展者体内病毒的序列特征和溯源分析

发布时间：2018-04-27 23:09

本文选题：艾滋病病毒型 + 长期无进展者　；参考：《中国艾滋病性病》2016年09期

【摘要】：目的分析感染艾滋病病毒1型(HIV-1)后长期无进展者体内病毒的序列特征,探讨影响艾滋病疾病进程的病毒学因素。方法HIV-1病毒载量用HIV-1 Monitor Version 1.5版本试剂在AmplicorCobas上检测;CD4+T淋巴细胞计数用multitest四色试剂在FASCalibur上进行计数;HIV-1近全长序列用三对引物扩增后进行测序,用ContigExpress软件进行序列编辑和拼接;HIV-1亚型用在线的REGA HIV-1Subtyping Tool-Version 3.0软件分析;HIV-1的溯源用BEAST软件包,采用贝叶斯马尔可夫链-蒙特卡罗(MCMC)算法构建系统进化树,推断该HIV-1毒株在中国最初的流行时间和地区;将HIV-1各蛋白的序列提交到至SWISS-MODEL蛋白质同源建模数据库(http://swissmodel.expasy.org/interactive),构建蛋白质结构。结果该感染者在潜伏期体内的病毒载量处于较低水平(4.32×103拷贝/mL),外周血中CD4+T淋巴细胞在感染10年后仍然高于500个/μL,该感染者感染的病毒为B亚型,最初于1992年左右在我国河南省流行。该病毒编码的Vpu与参考株HBX2编码的Vpu蛋白相比,结构存在显著的差异,这种差异可能影响该蛋白的功能。结论该感染者为长期无进展者,Vpu蛋白功能的部分丧失可能与疾病的长期无进展有关。
[Abstract]:Objective to investigate the virological factors influencing the progression of HIV / AIDS by analyzing the sequence characteristics of the virus in those who have no progress after HIV / AIDS (HIV 1) infection for a long time. Methods the viral load of HIV-1 was detected on AmplicorCobas with HIV-1 Monitor Version 1.5 reagents. The near-full-length sequence of HIV-1 was counted on FASCalibur using multitest four-color reagent. The sequence was sequenced by three pairs of primers. ContigExpress software was used for sequence editing and assembly of HIV-1 subtypes. The traceability of HIV-1 was analyzed by on-line REGA HIV-1Subtyping Tool-Version 3.0 software package. Bayesian Markov chain-Monte Carlo MCMCalgorithm was used to construct the phylogenetic tree. The initial epidemic time and region of the HIV-1 strain in China were inferred, and the sequence of HIV-1 proteins was submitted to the SWISS-MODEL protein homology modeling database, and the protein structure was constructed by using http: / swissmodel.expasy.org.interactive. Results the virus load in the latent period was 4.32 脳 10 ~ 3 copies / mL ~ (-1). The CD4 T lymphocytes in peripheral blood were still higher than 500 / 渭 L 10 years after infection. The virus infected by this infection was B subtype. It was first popular in Henan Province in 1992. Compared with the Vpu protein encoded by reference strain HBX2, the Vpu encoded by the virus has a significant difference in structure, which may affect the function of the protein. Conclusion the partial loss of Vpu protein function may be related to the long-term no-progression of the disease.
【作者单位】：江苏省疾病预防控制中心;江苏省血吸虫病防治研究所;
【基金】：江苏省卫生厅科教兴卫工程重点人才课题(RC2011083)~~
【分类号】：R512.91

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