重庆地区耐多药结核分枝杆菌基因分型特征分析

发布时间：2018-05-03 09:49

本文选题：结核分枝杆菌 + 耐多药　；参考：《第三军医大学学报》2017年12期

【摘要】：目的对重庆地区耐多药结核患者结核分枝杆菌进行基因分型,明确该地区基因类型的特征和流行情况。方法连续收集重庆市公共卫生医疗救治中心和重庆市第十二人民医院结核内科2013年7月至2015年3月753例临床诊断为耐多药结核患者的痰液或其他体液,采用液体培养法分离培养分枝杆菌,采用PCR方法对分枝杆菌分离株进行菌种鉴定,并使用多位点数目可变串联重复序列分析(multiple locus variable number of tandem repeat analysis,MLVA)方法进行基因分型。采用Bionumerics Version 3.0和phyloviz软件进行各基因位点的多态性和成簇分析。结果共分离培养出538例耐多药分枝杆菌菌株,经PCR鉴定确认结核分枝杆菌有503例(95.8%),非结核分枝杆菌有35例(4.2%)。北京家族型470例(93.4%,470/503),非北京家族型33例(6.6%,33/503)。503例分枝杆菌菌株共分为348个基因型,其中267例患者分离株为单一基因型,其余236例菌株可归入81个簇,成簇率为30.8%。北京家族型有76个簇,成簇率为30.8%,成簇比例为35.9%,非北京家族型有5个簇,成簇率为30.3%,成簇比例为33.3%。不同特征的耐多药结核人群对结核分枝杆菌北京基因型菌株易感因素分析:感染北京基因型菌株与性别无显著性关联(χ~2=2.68,P0.05);与年龄呈显著性关联(χ~2=784.00,P0.05)。结论重庆地区耐多药结核分枝杆菌的基因型存在明显的多态性,北京基因型占绝对优势;不同特征耐多药结核人群感染北京基因型菌株与性别无关,与年龄相关。
[Abstract]:Objective to study the genotyping of Mycobacterium tuberculosis in multidrug resistant tuberculosis (MDR-TB) patients in Chongqing. Methods from July 2013 to March 2015, 753 cases of sputum or other body fluids were collected from Chongqing Public Health Medical treatment Center and Department of Tuberculosis, 12th people's Hospital of Chongqing, which were clinically diagnosed as MDR-TB. Mycobacterium was isolated and cultured by liquid culture method, identified by PCR method, and genotyped by multilocus variable tandem repeat sequence analysis and multiple locus variable number of tandem repeat analysis (MLVA) method. Bionumerics Version 3.0 and phyloviz software were used to analyze the polymorphism and clustering of gene loci. Results A total of 538 strains of Mycobacterium multidrug resistance were isolated and cultured. By PCR identification, 503 cases of Mycobacterium tuberculosis and 35 cases of non-Mycobacterium tuberculosis were identified as Mycobacterium tuberculosis. 470 strains of Beijing family type were divided into 348 genotypes, 267 of them were single genotypes, and the remaining 236 strains could be classified into 81 clusters, with a clustering rate of 30.8. There are 76 clusters in Beijing family type with a clustering rate of 30.8 and a cluster ratio of 35.9.The non-Beijing family type has 5 clusters with a clustering rate of 30.3 and a cluster ratio of 33.3. Analysis of susceptibility to Mycobacterium tuberculosis strains of Beijing genotype in the population with different characteristics of MDR-TB: there was no significant correlation between Beijing genotype strains and sex (蠂 ~ (2 +) 2.68) (P0.05), but there was a significant correlation with age (蠂 ~ (2) 784.00 (P0.05). Conclusion the genotype of multidrug resistant Mycobacterium tuberculosis in Chongqing area is obviously polymorphic, and Beijing genotype is the dominant genotype, and the infection of Beijing genotype in different characteristics of MDR-TB population is not related to sex, but is related to age.
【作者单位】：重庆市公共卫生医疗救治中心检验科;重庆市第十二人民医院检验科;重庆市公共卫生医疗救治中心耐多药结核病管理办公室;重庆市公共卫生医疗救治中心结核重点实验室;重庆市公共卫生医疗救治中心病案室;重庆医科大学附属第三医院骨与创伤中心;
【基金】：重庆市卫生局2012年医学科研重点项目(2012-1-085)~~
【分类号】：R440;R52

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