应用基质辅助激光解吸电离飞行时间质谱研究细菌性血流感染的血清多肽指纹图谱

发布时间：2018-05-04 20:42

本文选题：基质辅助激光解吸电离飞行时间质谱(MALDI-TOF + MS)　；参考：《解放军医学杂志》2017年05期

【摘要】：目的应用基质辅助激光解吸电离飞行时间质谱系统(MALDI-TOF MS)分析金黄色葡萄球菌、大肠埃希菌引起的血流感染血清多肽指纹图谱,寻找差异多肽峰并建立相应的诊断模型。方法建立ICR小鼠金黄色葡萄球菌、大肠埃希菌血流感染模型,收集血清标本,经弱阳离子交换磁珠纯化,MALDI-TOF MS检测及BioExplorer生物软件分析研究金葡菌感染组与正常对照组、大肠埃希菌感染组与正常对照组间血清多肽指纹图谱。结果比较金葡菌感染组与正常对照组发现,表达上调的多肽有6个,表达下调的多肽有7个,表达呈先上调后下调的多肽有8个。比较大肠埃希感染组与正常对照组发现,表达下调的多肽有5个,表达呈先下调后上调的多肽有4个,表达呈先上调后下调的多肽有8个。结论利用MALDI-TOF MS技术和Bio Explorer软件研究大肠埃希菌和金黄色葡萄球菌血流感染的血清多肽,可以发现感染组与正常对照组间的差异多肽并有效地建立这两种细菌感染的诊断模型,对于细菌性血流感染的诊断具有一定的价值。
[Abstract]:Objective to analyze the serum polypeptide fingerprints of Staphylococcus aureus and Escherichia coli by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF), and to search for the differential peptide peaks and to establish a diagnostic model. Methods the blood stream infection model of Staphylococcus aureus and Escherichia coli in ICR mice was established, and the serum samples were collected. MALDI-TOF MS detection and BioExplorer software analysis were used to study the infection of Staphylococcus aureus and normal control group. Serum polypeptide fingerprints between Escherichia coli infection group and normal control group. Results compared with the control group, there were 6 up-regulated polypeptides, 7 down-regulated peptides and 8 down-regulated polypeptides in the Staphylococcus aureus infection group. Compared with the control group, there were 5 down-regulated peptides, 4 down-regulated peptides and 8 down-regulated peptides. Conclusion using MALDI-TOF MS and Bio Explorer software to study the serum polypeptides of Escherichia coli and Staphylococcus aureus blood stream infection, we can find the different peptides between the infected group and the normal control group and establish the diagnosis model of these two bacterial infections effectively. It has certain value for the diagnosis of bacterial blood flow infection.
【作者单位】：温州医科大学检验医学院生命科学院;解放军总医院临床检验科;
【分类号】：R446.1;R515

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