HPV女性感染者男性性伴侣HPV感染与免疫功能关系研究

发布时间：2018-05-07 14:34

本文选题：人乳头瘤病毒 + 男性　；参考：《蚌埠医学院》2017年硕士论文

【摘要】：目的:研究女性HPV感染者的男性性伴侣病毒HPV感染状况及其外周血中淋巴细胞亚群、免疫球蛋白和补体含量变化,从而为阻断HPV传播及相关疾病的预防和免疫治疗提供依据。方法:选择110例女性HPV感染者的男性性伴侣作为研究对象,取其尿道口上皮细胞及空腹外周血。所有研究对象均需签署知情同意书。采用PCR-反向点杂交法进行HPV分型检测,以流式细胞仪进行淋巴细胞亚群检测(CD3+、CD4+、CD8+、CD4+/CD8+、CD16+56+、CD19+细胞),以特种蛋白分析仪免疫散射比浊法检测免疫球蛋白(IgA、IgG、IgM)及补体(C3、C4)含量。使用SPSS19.0版软件对数据进行统计学分析。结果:1.女性单一亚型感染占61.82%(68/110),多重感染占38.18%(42/110)。110例女性HPV感染者的男性性伴侣的感染率为感染率33.64%(37/110),其中单一亚型感染占62.16%(23/37),多重感染占37.84%(14/37)。2.男女高低危HPV亚型出现频次有显著性统计学差异(X2=4.040,P=0.0440.05)。3.性伴双方HPV吻合率为54.05%(20/37)。4.无症状男性HPV阴性组与阳性组外周血免疫指标比较,差异无统计学意义(P均0.05)。5.HPV高危型组和低危型组CD8+T细胞、CD4+/CD8+T细胞比值均有显著性统计学差异(P均0.05);多重高危型感染者外周血细胞免疫指标变化较大;多重高危型HPV感染(1.50±0.42)较单一低危型感染(0.76±0.27)IgM含量有显著性差异(P0.05)。6.免疫指标正常组和异常组比较HPV感染率无统计学差异(P0.05)。结论:1.该地区HPV感染的女性及其男性性伴,双方HPV感染以单一亚型、高危亚型为主;女性对高危型HPV易感性高于男性,而男性对低危型HPV的易感性高于女性;HPV在性伴中传播,控制男性HPV感染,减少HPV在性伴侣之间的循环感染,具有重要意义。2.高危型HPV无症状男性感染者外周血免疫功能主要趋向于细胞免疫抑制状态,多重高危型感染尤为明显;血清IgM可能在HPV感染早期发挥重要作用。
[Abstract]:Objective: to study the status of male sexual chaperone virus (HPV) infection and the changes of lymphocyte subsets, immunoglobulin and complement levels in peripheral blood of female patients with HPV, so as to provide evidence for blocking the transmission of HPV and the prevention and immunotherapy of related diseases. Methods: the male sexual partners of 110 women with HPV infection were selected as the study subjects, and their urethral orifice epithelial cells and fasting peripheral blood were collected. All subjects are required to sign informed consent. HPV typing was carried out by using PCR- reverse dot hybridization, lymphocyte subsets were detected by flow cytometry (FCM), CD4 / CD8 / CD8 / CD1656 / CD19 cells were detected by flow cytometry, and the immunoglobulin IgA (IgA) and complement C _ (3) C _ (4) were detected by immunosurgery and turbidimetry by special protein analyzer. Use the SPSS19.0 version of the software for statistical analysis of the data. The result is 1: 1. Female single subtype infection accounted for 61.82% 68 / 110, multiple infection accounted for 38.18% 42 / 110% male sexual partner infection rate was 33.64% 37 / 110%, among them, single subtype infection accounted for 62.16% 23 / 37%, multiple heavy infection accounted for 37.84% 14 / 37.2. There was significant statistical difference in the frequency of HPV subtypes in male and female patients with high and low risk. The rate of HPV anastomosis was 54.05%, 20% 37%. 4%. The peripheral blood immunological indexes of asymptomatic male HPV negative group and positive group were compared. There was no significant difference in the ratio of CD4 / CD8 T cell between 0.05).5.HPV high risk group and low risk group. There was significant difference in the content of 0.76 卤0.27)IgM between multiple high risk type HPV infection and single low risk type infection (P 0.05 卤0.42). There was no significant difference in HPV infection rate between normal group and abnormal group (P 0.05). Conclusion 1. In this area, women with HPV infection and their male sexual partners had a single subtype and high risk subtype of HPV infection, while women were more susceptible to high risk HPV than men, and men were more susceptible to low risk HPV than women. It is important to control male HPV infection and reduce HPV circulating infection between sexual partners. The immune function of peripheral blood of high risk HPV asymptomatic male patients tended to be mainly cellular immunosuppressive, especially multiple high risk type infection, and serum IgM may play an important role in the early stage of HPV infection.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R511

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