HBsAg定量与HBeAg、HBVDNA、基因型的相关性研究

发布时间：2018-05-08 00:02

  本文选题：乙型肝炎表面抗原 + 乙型肝炎E抗原　； 参考：《苏州大学》2014年硕士论文

【摘要】：目的：探讨HBsAg定量与HBeAg、HBVDNA、HBV基因型的相关性。 方法：收集2010年12月至2013年12月在泰兴市人民医院确诊的154例乙肝病例,既往均未使用过抗乙肝病毒药物。男113例,女41例,中位年龄38(17-70)岁。其中慢性乙型肝炎130例(轻度43例,中度47例,重度40例),肝硬化24例；HBeAg阳性127例,HBeAg阴性27例；B基因型48例,C基因型104例,非B非C基因型2例。采用电化学发光法检测HBsAg、 HBeAg定量,荧光定量PCR法测定HBVDNA定量、HBV基因型进行统计学分析。 结果：1.HBsAg定量与HBeAg定量及HBVDNA定量均呈负相关关系(r=-0.42,P0.05:r=0.43,P0.05)。2.B基因型患者HBsAg定量与HBeAg定量呈负相关关系(r=-0.46,P0.05)、与HBVDNA定量无相关关系(r=-0.30,P0.05),C基因型患者HBsAg定量与HBeAg定量呈负相关关系(r=-0.39,P0.05)、与HBVDNA定量无相关关系(r=一0.24,P0.05)。3.在B、C基因型患者中,HBsAg定量分别为3767.4±2257.1COI,3548.2±2339.9COI,两者相比差异无统计学意义(t=0.550, P=0.584)；HBeAg定量分别为437.6±452.5COI,521.4±438.6COI,两者相比差异无统计学意义(t=1.072,P=0.287)；HBVDNA定量分别为6.7±1.410gIU/ml,6.7±1.2logIU/ml,两者相比差异无统计学意义(t=-0.204,P=0.839)。4.与HBeAg阴性患者比较,HBeAg阳性患者血清HBVDNA定量明显增高(t=2.687,P0.05)。 结论：HBsAg定量与HBeAg定量、HBVDNA定量呈负相关,与HBV基因型无相关。不同基因型之间HBsAg定量、HBeAg定量、HBVDNA定量无统计学差异。HBeAg阳性患者血清HBVDNA定量显著高于HBeAg阴性患者。
[Abstract]:Objective: to investigate the relationship between HBsAg quantification and HBV genotype in HBeAgN HBV DNA. Methods: 154 cases of hepatitis B diagnosed in Taixing people's Hospital from December 2010 to December 2013 were collected. There were 113 males and 41 females with a median age of 3817-70 years. There were 130 cases of chronic hepatitis B (mild 43 cases, moderate 47 cases, severe 40 cases), 24 cases of liver cirrhosis with HBeAg positive 127 cases with HBeAg negative 27 cases with HBeAg genotype 48 cases with C genotype and 104 cases with non-B non-C genotype (104 cases). HBsAg and HBeAg genotypes were detected by electrochemiluminescence assay and HBVDNA genotypes were detected by fluorescence quantitative PCR method. Results 1. There was a negative correlation between HBsAg quantification and HBeAg quantification and HBVDNA quantification. There was a negative correlation between HBsAg quantification and HBeAg quantification in patients with genotype HBeAg, but no correlation between HBsAg quantification and HBeAg quantification in HBVDNA genotype patients. There was a negative correlation between HBsAg quantification and HBeAg quantification in patients with genotype HBVDNA, and there was a negative correlation between HBsAg quantification and HBeAg quantification in patients with genotype B, and there was a negative correlation between HBsAg quantification and HBeAg quantification in patients with genotype HBeAg, and there was a negative correlation between HBsAg quantity and HBeAg quantification in patients with genotype HBeAg. There was no correlation between HBVDNA quantification and r = -0.24 (P0.05N. 3). The quantification of HBVDNA in the patients with genotype B was 3767.4 卤2257.1COI 3548.2 卤2339.9COI.There was no significant difference between the two groups. There was no significant difference between the two groups (437.6 卤452.5COI, 521.4 卤438.6COI, respectively). There was no significant difference in HBVDNA between the two groups (6.7 卤1.410g / ml vs 6.7 卤1.2logIUP / ml, respectively). There was no significant difference between the two groups. Compared with HBeAg negative patients, the serum HBVDNA of HBeAg positive patients was significantly higher than that of HBeAg negative patients. Conclusion there was a negative correlation between HBeAg DNA and HBeAg quantification, but no correlation with HBV genotypes. There was no statistical difference between different genotypes. The serum HBVDNA quantity of HBeAg positive patients was significantly higher than that of HBeAg negative patients.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R512.62

【共引文献】

相关硕士学位论文 前3条

1 俞立飞;替比夫定治疗HBeAg阳性慢性乙型肝炎患者e抗原血清学转换的基线预测因素分析[D];浙江大学;2014年

2 潘月;内质网分子伴侣PDIA3在非酒精性脂肪性肝病中的作用及机制研究[D];浙江大学;2014年

3 曾林燕;浙江省慢性HBV感染者中乙型肝炎病毒表面抗原（HBsAg）水平的横断面研究[D];浙江大学;2014年

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