2015年湖北地区诺如病毒流行特点及基因型别特征

发布时间：2018-05-08 21:53

本文选题：诺罗病毒 + 流行病学　；参考：《中华疾病控制杂志》2017年04期

【摘要】：目的了解湖北地区腹泻患者诺如病毒(norovirus,NV)的流行病学特征及基因型别特征。方法收集2015年1~12月儿童和成人腹泻患者的粪便标本687份,采用Real-time逆转录聚合酶链式反应(reverse transcription-polymerase chain reavtions,RT-PCR)方法进行NV的核酸检测;应用RT-PCR方法对NV检测阳性的GII型标本再行扩增ORF 2基因N/S区基因片段后,将该PCR产物直接测序并进行基因型别和系统发育分析。结果687份腹泻患者粪便标本中男性感染者93例,女性感染者56例,性别差异无统计学意义(x~2=1.193,P=0.275)。患者主要为5岁及以下儿童。2015年湖北地区NV感染全年均有发生,但流行高峰出现在9月份;NV主要为GII基因型,仅检出2例GI基因型。系统发育分析显示,NV GII流行株可分为5种基因亚型,分别为GII.17、GII.4 Sydney 2012、GII.3、GII.6和GII.13,不同基因亚型的流行呈现月份更替现象。结论 2015年湖北地区存在多种基因型NV的感染,加强NV的分子流行病学监测有利于NV的科学防控。
[Abstract]:Objective to investigate the epidemiological and genotypic characteristics of norovirus NVV in patients with diarrhea in Hubei province. Methods A total of 687 fecal specimens from children and adults with diarrhea were collected from January to December 2015. The nucleic acid of NV was detected by reverse transcription-polymerase polymerase chain reaction (Real-time) reverse transcription-polymerase chain reams RT PCR. The RT-PCR method was used to amplify the N / S region of the ORF 2 gene in the NV positive GII samples. The PCR product was sequenced directly and genotypic and phylogenetic analysis were performed. Results among 687 fecal specimens of diarrhea patients, 93 were male and 56 were female. There was no significant difference between male and female. NV infection occurred all year round in Hubei province in 2015, but the epidemic peak occurred in September. Only 2 cases of GI genotype were detected. Phylogenetic analysis showed that the epidemic strains of NV GII could be divided into five subtypes, namely GII.17GII.4 Sydney 2012GII.3GII.6 and GII.13. the prevalence of different gene subtypes showed the phenomenon of month turnover. Conclusion there are many genotypes of NV infection in Hubei province in 2015. Strengthening the molecular epidemiological monitoring of NV is beneficial to the prevention and control of NV.
【作者单位】：湖北省疾病预防控制中心卫生检验所;宜昌市疾病预防控制中心病原微生物检验所;襄阳市疾病预防控制中心急传科;恩施市疾病预防控制中心急传科;汉川市疾病预防控制中心急传科;
【分类号】：R512.5;R440

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