IL-28B血清水平和遗传变异与慢性丙型肝炎患者病毒基因型及疾病进展相关性分析

发布时间：2018-05-11 02:41

本文选题：IL-B + 基因单核苷酸多态性　；参考：《中华医院感染学杂志》2017年09期

【摘要】：目的探讨IL-28B血清水平和单核苷酸多态性(SNP)与丙型肝炎患者病毒基因型及疾病进展相关性。方法选取2009年1月-2015年12月医院收治的258例慢性丙型肝炎(慢性丙型肝炎组)、55例丙型肝炎肝硬化(丙型肝炎肝硬化组)和31例丙型肝炎肝癌(肝癌组)患者及223例健康体检者(对照组)为研究对象,采用TaqMan@SNP Genotyping Assay和实时定量聚合酶链式反应检测IL-28BSNPs基因型;运用ELISA检测血清IL-28B浓度;稳健回归和ROC曲线分析血清IL-28B水平和SNPs的诊断价值。结果丙型肝炎患者6个IL-28BSNP基因频率与健康体检者比较,差异有统计学意义(P0.05);慢性丙型肝炎患者血清IL-28B水平(112.8±40.9)pg/ml显著低于丙型肝炎肝硬化患者(354.8±40.3)pg/ml及丙型肝炎肝硬化+肝癌患者(302.5±50.1)pg/ml,(P0.01);患者中rs8099917TT携带者血清IL-28B水平(147.8±40.5)pg/ml显著低于TG携带者(295.8±50.2)pg/ml,(P0.05);稳健回归发现血清IL-28B水平与rs8099917TG、慢性丙型肝炎、丙型肝硬化+肝癌密切相关(P均0.05);ROC分析239pg/ml血清IL-28B为慢性丙型肝炎和丙型肝炎肝硬化+肝癌界值。结论血清IL-28B可作为丙型肝炎病毒(HCV)疾病进展的辅助诊断指标。
[Abstract]:Objective to investigate the relationship between serum level and single nucleotide polymorphism (SNP) of IL-28B and HCV genotype and disease progression in patients with hepatitis C. Methods from January 2009 to December 2015, 258 patients with chronic hepatitis C (chronic hepatitis C group), 55 patients with cirrhosis of hepatitis C (cirrhosis of hepatitis C) and 31 patients with liver cancer of hepatitis C (liver cancer group) and 223 patients with liver cancer were enrolled in our hospital from January 2009 to December 2015. Cases of healthy persons (control group) were studied. TaqMan@SNP Genotyping Assay and real-time quantitative polymerase chain reaction (RQPCR) were used to detect IL-28BSNPs genotypes, ELISA was used to detect serum IL-28B concentration, and robust regression and ROC curves were used to analyze the serum IL-28B level and the diagnostic value of SNPs. Results the frequencies of 6 IL-28BSNP genes in patients with hepatitis C were compared with those in healthy controls. The levels of serum IL-28B in patients with chronic hepatitis C were significantly lower than those in patients with hepatitis C cirrhosis (354.8 卤40.3)pg/ml) and patients with liver cirrhosis of hepatitis C (302.5 卤50.1 mg / ml), and the levels of serum IL-28B in rs8099917TT carriers were significantly lower than those in patients with TG. The serum IL-28B level and rs8099917 TG, chronic hepatitis C, were found by robust regression. The serum IL-28B of 239pg/ml was determined to be the boundary value of liver cancer of chronic hepatitis C and hepatitis C cirrhosis. Conclusion Serum IL-28B can be used as an auxiliary diagnostic index for the progression of hepatitis C virus (HCV).
【作者单位】：西安医学院第二附属医院检验科;西北妇女儿童医院检验科;西安医学院第二附属医院医院控感科;
【分类号】：R446.6;R512.63

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