2010-2014年吉林省流感病原学监测分析

发布时间：2018-05-12 02:02

本文选题：流感 + 人　；参考：《中华疾病控制杂志》2015年10期

【摘要】：目的了解吉林省流感病毒的流行病学特征,为流感防控措施的制定提供科学依据。方法采集哨点医院流感样病例的鼻咽拭子标本,用实时荧光定量PCR方法进行病毒核酸检测,流感病毒核酸阳性标本接种马丁达比犬肾细胞(madin-daiby canine kidney cells,MDCK)细胞进行病毒分离,对分离后的毒株用红细胞凝集试验(hemagglutination test,HA)及红细胞凝集抑制试验(hemagglutination inhibitoin test,HI)进行型别鉴定。结果 2010-2014年监测年度报告的流感样病例比例差异有统计学意义(X~2=8 969.11,P0.001)。2010-2014年监测年度共采集流感样病例患者鼻咽拭子标本22 703份,分离得到流感病毒毒株共1 103株,总毒株分离率为4.86%。2011-2012年以B型流感病毒为流行优势株,其他三个监测年度都以A型流感病毒为流行优势株,流感流行优势株的具体型别与全国其他省份的流感流行优势株基本一致。结论 2010-2014年流感样病例就诊比例有逐年升高的趋势,主要感染人群是儿童和少年,应加强相应的防控措施并积极推广流感疫苗的接种。
[Abstract]:Objective to understand the epidemiological characteristics of influenza virus in Jilin Province and to provide scientific basis for the formulation of influenza prevention and control measures. Methods the nasopharyngeal swabs of influenza like patients in sentinel hospitals were collected, and the viral nucleic acid was detected by real-time fluorescence quantitative PCR. The positive samples of influenza virus nucleic acids were inoculated into Martin Darby's kidney cells, and the virus was isolated from Martin daiby canine kidney cells MDCK cells. The type of the isolated strain was identified by hemagglutination test (HA) and hemagglutination inhibitoin test (HI). Results there was a statistically significant difference in the proportion of influenza-like cases reported in the surveillance year 2010-2014. A total of 22,703 specimens of nasopharyngeal swabs were collected from patients with influenza-like cases in 2010-2014. A total of 1,103 strains of influenza viruses were isolated. The total virus isolation rate was 4.86.Influenza B was the dominant strain in 2011-2012, and influenza A was the dominant strain in the other three surveillance years. The specific types of influenza epidemic dominant strains are basically the same as those of other provinces in China. Conclusion from 2010 to 2014, the proportion of influenza-like cases is increasing year by year, the main infected population is children and adolescents, we should strengthen the corresponding prevention and control measures and actively promote influenza vaccination.
【作者单位】：吉林省疾病预防控制中心病毒检验所;
【分类号】：R511.7;R440

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