水痘-带状疱疹病毒感染人神经母细胞瘤细胞的形态观察

发布时间：2018-05-12 11:44

本文选题：水痘-带状疱疹病毒 + SHSYY细胞　；参考：《中国皮肤性病学杂志》2017年02期

【摘要】：目的研究水痘-带状疱疹病毒(VZV)在人神经母细胞瘤细胞株(SHSY5Y)中培养及增殖的特点,观察VZV感染SHSY5Y细胞后的生物学效应。方法于普通倒置显微镜和电子显微镜下观察被感染细胞的形态变化;利用CCK-8法检测VZV感染对细胞增殖的影响;比较不同感染复数(MOI)和不同浓度胎牛血清维持液对病毒增殖的影响。结果普通倒置显微镜下观察细胞病变效应(cytopathic effect,CPE),从24h~96h,细胞病变数量增加和病变程度加重。电镜观察显示:感染48 h后核染色质固缩、浓染,线粒体肥大及增生,胞质空泡化和自噬小体形成,可见包装成熟的病毒颗粒。CCK-8法提示VZV感染24h后细胞增殖被抑制,各实验组存活率与对照组比较差异均有统计学意义(P0.05)。在2%胎牛血清的维持液中用MOI为1的VZV感染SHSY5Y细胞,能收获较高滴度的病毒。结论初步建立了VZV感染SHSY5Y细胞的体外模型,并利用该模型观察病毒感染对细胞生物性状的影响,为探讨VZV的致病及潜伏机制奠定了基础。
[Abstract]:Objective to study the characteristics of the culture and proliferation of varicella-zoster virus (VZV) in human neuroblastoma cell line SHSY5Y, and to observe the biological effects of VZV infection on SHSY5Y cells. Methods the morphological changes of infected cells were observed under inverted microscope and electron microscope, and the effects of VZV infection on cell proliferation were detected by CCK-8 assay. The effects of different infective complex moi) and different concentrations of fetal bovine serum maintenance fluid on virus proliferation were compared. Results the cytopathic effect of cytopathic effect was observed under ordinary inverted microscope. From 24 h to 96 h, the number of cytopathic effect increased and the severity of cytopathic effect increased. Electron microscope observation showed that nuclear chromatin pyknosis, staining, hypertrophy and proliferation of mitochondria, vacuolation of cytoplasm and formation of autophagy were observed 48 h after infection. The survival rate of each experimental group was significantly different from that of the control group (P 0.05). In the maintenance solution of 2% fetal bovine serum, SHSY5Y cells were infected with VZV with MOI 1, and the higher titer of the virus was obtained. Conclusion the model of VZV infected SHSY5Y cells in vitro was established, and the effect of virus infection on cell biological characters was observed, which laid a foundation for the study of pathogenesis and latent mechanism of VZV.
【作者单位】：南方医科大学;广州军区广州总医院皮肤科;
【分类号】：R752.12

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