云南省曲靖市啮齿动物的立克次体血清学和分子流行病学调查

发布时间：2018-05-13 09:06

本文选题：啮齿动物 + 立克次体　；参考：《中国人兽共患病学报》2015年01期

【摘要】：目的调查云南省曲靖市啮齿动物感染立克次体状况。方法用鼠笼法捕鼠,采集鼠血清和脾脏。用间接免疫荧光试验(IFA)检测鼠血清中7种常见立克次体的IgG抗体;用巢氏PCR方法检测鼠脾中嗜单核细胞埃立克体和嗜吞噬细胞无形体16SrRNA基因片段部份序列。结果 2012年7-9月在曲靖市捕获啮齿动物3种592只,褐家鼠(Rattus norvegicus)和黄胸鼠(Rattus flavipectus)构成比分别为61.49%和35.47%。鼠血清中贝氏柯克斯体(Coxiella burentii)、莫氏立克次体(Rickettsia typhi)、恙虫病东方体(Orientia tsutsugamushi)、西伯利亚立克次体(Rickettsia sibirica)、嗜吞噬细胞无形体(Anaplasma phagocytophilum)和嗜单核细胞埃立克体(Ehrlichia chaffeensis)的抗体阳性率依次为21.45%、7.60%、7.09%、3.38%、1.18%和0.51%;未检测到猫立克次体(Rickettsia felis)抗体。从5份褐家鼠脾脏标本中检测到16SrRNA基因序列,同源性和进化分析表明,1株为嗜吞噬细胞无形体,4株为莫氏立克次体。结论曲靖市啮齿动物中存在莫氏立克次体和人粒细胞无形体流行,褐家鼠可能是主要宿主;同时还存在恙虫病东方体、贝氏柯克斯体、斑点热立克次体和埃立克体的感染。当地疾控和医疗机构应加强立克次体病的监测。
[Abstract]:Objective to investigate the status of Rickettsia in rodent infection in Qujing, Yunnan province. Methods mouse squirrel cage method was used to capture rat serum and spleen. Indirect immunofluorescence test (IFA) was used to detect the IgG antibody of 7 common Rickettsia in rat serum, and the nesting PCR method was used to detect the eosinophil and phagocytic 16SrRNA base in the spleen. As a result of the fragment sequence, 3 rodent species were captured in Qujing in 7-9 months of 2012, and the composition ratio of Rattus norvegicus and Huang Xiongshu (Rattus flavipectus) was 61.49% and the serum of 35.47%. mice (Coxiella burentii), Moji Li (Rickettsia typhi), and the Oriental body of tsutsugamushi disease (Orientia tsutsug) Amushi), Siberia rickettsia (Rickettsia sibirica), the antibody positive rates of phagocytic cells (Anaplasma phagocytophilum) and eelictophils (Ehrlichia chaffeensis) were 21.45%, 7.60%, 7.09%, 3.38%, 1.18% and 0.51%, and no antibodies to the cat rickettsia (Rickettsia felis) were detected. From 5 mice of the spleen of norvegicus The 16SrRNA gene sequence was detected in the specimens, and the homology and evolutionary analysis showed that 1 were phagocytic and 4 were MMO Rickettsia. Conclusion the rodent Rickettsia and human granulocyte in Qujing rodents are not in form, and Rattus norvegicus may be the main host; meanwhile, the Oriental body of tsutsugamushi disease, bainite Kirk body, spots also exist. Infection of Rickettsia and Erik bodies should be noted. Surveillance of Rickettsia disease should be strengthened in local disease control and medical institutions.

【作者单位】：云南省地方病防治所/云南省病毒立克次体研究中心;中国疾病预防控制中心传染病预防控制中心;新疆石河子大学动物科技学院;曲靖市疾病预防控制中心;
【基金】：国家基础研究973项目(2010CB530206;2012CB722501)~~
【分类号】：R513

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