马尔尼菲青霉菌对支气管上皮细胞吞噬和细胞因子分泌的影响

发布时间：2018-05-16 07:08

本文选题：马尔尼菲青霉菌 + 支气管上皮细胞　；参考：《中华医院感染学杂志》2017年02期

【摘要】：目的探讨马尔尼菲青霉菌(PM)孢子对人支气管上皮细胞BEAS-2B吞噬和细胞因子分泌的影响。方法人HIV-分离株孢子与细胞培养6hPAS染色,观察孢子黏附细胞的情况;HIV-分离株孢子与细胞培养4h行荧光原位杂交(FISH),观察细胞吞噬孢子的情况;HIV-分离株孢子与细胞培养1~5h,透射电镜观察细胞吞噬孢子的过程;(人HIV-分离株孢子、人HIV+分离株孢子、FRR2161孢子、野生分离株孢子、及LPS分别加入以上4种分离株孢子、空白对照及LPS阳性对照组)10组实验组分别与细胞培养24、48、72h,用ELISA测定细胞上清液IL-6和IL-8浓度。结果 PAS染色示孢子紧紧黏附细胞;FISH示细胞能吞噬孢子;透射电镜示细胞吞噬孢子后,孢子细胞壁被破坏,细胞溶酶体增多和线粒体空泡化并轻度肿胀;3个时间段的LPS阳性对照组、孢子刺激组及孢子加LPS刺激组的细胞上清液IL-6及IL-8浓度均高于空白对照组(P0.05)。结论 PM孢子与BEAS-2B细胞发生黏附后被吞噬,细胞内溶酶体将孢子消化破坏,并导致细胞分泌的IL-6和IL-8浓度增加。
[Abstract]:Objective to investigate the effect of Penicillium marneffei spores on BEAS-2B phagocytosis and cytokine secretion in human bronchial epithelial cells. Methods the spore and cell culture of human HIV-1 isolate were stained with 6hPAS. To observe the adherence of spores to cells and to observe the phagocytosis of the spores of HIV-isolated strains by fluorescence in situ hybridization for 4 h, and to observe the process of phagocytosis of spores by transmission electron microscope (TEM). Human HIV-isolate spores, Human HIV isolates FRR2161 spores, wild isolates spores, and LPS were added to the above four isolates respectively. The 10 experimental groups of blank control group and LPS positive control group were cultured with the cells for 24: 48 and 72 h, respectively. The concentration of IL-6 and IL-8 in the supernatant of cells was measured by ELISA. Results PAS staining showed that the cells could be phagocytosed by fish, and the cell walls were destroyed by transmission electron microscopy. The concentrations of IL-6 and IL-8 in the supernatant of LPS positive control group, spore stimulation group and spore plus LPS group were higher than those in the blank control group (P 0.05). Conclusion PM spores are adhered to BEAS-2B cells and then phagocytized. Lysosomes in the cells will destroy the spores and increase the concentration of IL-6 and IL-8 secreted by the cells.
【作者单位】：广西医科大学第一附属医院呼吸内科;
【分类号】：R519

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