Fonsecaea monophora所致皮肤着色芽生菌病3例病原学鉴定及临床分析

发布时间：2018-05-16 09:45

  本文选题：Fonsecaea + monophora　； 参考：《中国皮肤性病学杂志》2015年08期

【摘要】：目的对3例Fonsecaea monophora所致皮肤着色芽生菌病进行病原学鉴定及临床分析。方法分析3例患者的临床特点。取患者皮损脓液直接镜检,皮损组织病理学检查及真菌培养。提取病原菌DNA,利用引物ITS4和ITS5进行r DNA ITS区序列的PCR扩增,扩增产物测序后在Gen Bank核酸数据库及CBS数据库中进行同源性比对。结果皮损组织病理表现为慢性肉芽肿样改变,并可见硬壳小体。真菌培养可见暗棕色、橄榄色至黑色菌落生长,生长速度较慢,镜下可见枝孢型和喙枝孢型产孢。温度实验37℃生长,38℃不生长。0.01%,0.05%和0.1%放线菌酮均能耐受。经序列分析与Gen Bank及CBS数据库中Fonsecaea monophora的ITS区进行同源性比对,其同源性高。结合3例患者组织病理、真菌学特征及r DNA ITS区序列分析将病原菌鉴定为Fonsecaea monophora。确诊后经口服伊曲康唑200mg,2次/d,6~8周后以200mg,1次/d维持治疗,或特比萘芬250mg,1次/d。治疗3个月后,皮损逐渐消退。结论 Fonsecaea monophora与裴氏着色霉相似,可引起皮肤着色性芽生菌病,可通过分子生物学方法鉴别,口服伊曲康唑或特比萘芬治疗有效。
[Abstract]:Objective to identify and analyze the etiology of 3 cases of dermatochromocytoma caused by Fonsecaea monophora. Methods the clinical features of 3 patients were analyzed. Direct microscopic examination, histopathological examination and fungal culture were performed. The ITS4 and ITS5 primers were used to amplify the r DNA ITS region by PCR. The amplified products were sequenced and compared with each other in the Gen Bank nucleic acid database and CBS database. Results the pathological features of the lesions were chronic granulomatous changes, and hard shell bodies could be seen. Fungus culture showed dark brown, olive-color to black colony growth, the growth rate was slow, under the microscope can be seen in the cytosporium type and the beak Cladosporium type sporulation. In the temperature experiment, 37 鈩，

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