肺结核患者外周血中Treg细胞和Th17细胞等4种T细胞亚群与其发病的关系

发布时间：2018-05-16 15:23

本文选题：肺结核 + Treg细胞　；参考：《国际检验医学杂志》2016年24期

【摘要】：目的通过检测肺结核患者和健康人群外周血中Treg细胞、Th17细胞以及CD8~+CD28~+T细胞和CD8~+CD28-T细胞的数量,探讨各细胞亚群的数量与肺结核发病的关系,为结核的诊断、治疗及预后提供新思路。方法应用流式细胞技术检测Treg细胞、Th17细胞以及CD8~+CD28~+T细胞和CD8~+CD28-T细胞,分别比较肺结核组、初治组、复治组与健康对照组之间的上述指标是否存在差异。结果 (1)肺结核组、初治组及复治组外周血中Th17细胞的数量分别为(0.74±0.33)%、(0.76±0.34)%、(0.73±0.32)%,CD8~+CD28~+T细胞的数量分别为(39.5±11.4)%、(39.5±10.9)%、(38.7±12.2)%,均分别低于健康对照组(1.17±0.38)%、(62.5±12.0)%,差异有统计学意义(P0.01);(2)肺结核组、初治组及复治组外周血中Treg细胞的数量分别为(10.1±2.7)%、(11.4±3.8)%、(9.4±2.5)%,CD8~+CD28-T的数量分别为(60.5±11.4)%、(60.5±10.9)%、(61.3±12.2)%,均分别高于健康对照组的(7.2±2.2)%、(37.5±12.0)%,差异有统计学意义(P0.01);(3)初治组患者与复治组患者外周血中Th17细胞、Treg细胞、CD8~+CD28~+T细胞、CD8~+CD28-T细胞数量之间的差异无统计学意义(P0.05)。结论肺结核患者外周血中的效应性T细胞亚群Th17细胞和CD8~+CD28~+T细胞下降,具有抑制功能的CD4~+CD25~+CD127low/-Treg细胞和CD8~+CD28-T细胞升高,以上4个T细胞亚群可能与肺结核的发病过程相关。
[Abstract]:Objective to investigate the relationship between the number of Treg Th17 cells, CD8 ~ CD28 ~ T cells and CD8 ~ CD28-T cells in peripheral blood of pulmonary tuberculosis patients and healthy people, and to investigate the relationship between the number of subsets and the incidence of tuberculosis. Treatment and prognosis provide new ideas. Methods the Th17 cells, CD8 ~ CD28 ~ T cells and CD8 ~ CD28-T cells of Treg cells were detected by flow cytometry, and the above indexes were compared between the pulmonary tuberculosis group, the initial treatment group, the retreated group and the healthy control group. Results (1) the number of Th17 cells in peripheral blood of pulmonary tuberculosis group, primary treatment group and recurrent group were 0.74 卤0.33 and 0.76 卤0.34 respectively. The number of CD8 ~ CD28 ~ T cells in pulmonary tuberculosis group was 39.5 卤11.4 卤10.97.78.7 卤12.2T, respectively, which was significantly lower than that in healthy control group (1.17 卤0.3862.5 卤12.0). The number of Treg cells in peripheral blood of primary treatment group and retreated group were 11.4 卤3.8 and 9.4 卤2.5 respectively. The numbers of CD8 ~ CD28-T were 60.5 卤11.4 卤10.9 and 61.3 卤12.2 respectively, which were higher than those of healthy control group (7.2 卤2.22 卤12.0). The difference was statistically significant (P 0.01). There was no significant difference in the number of CD8 ~ CD28-T cells between CD8 ~ CD28 ~ T cells. Conclusion the effectual T cell subsets Th17 and CD8 ~ CD28 ~ T cells in peripheral blood of pulmonary tuberculosis patients decreased, but the inhibitory CD4 ~ CD25 ~ CD127low/-Treg cells and CD8 ~ CD28-T cells increased. The above four T cell subsets may be related to the pathogenesis of pulmonary tuberculosis.
【作者单位】：贵州医科大学附属医院中心实验室;贵州省贵阳市第一人民医院检验科;
【分类号】：R521

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