广东省含麻疹成分疫苗接种后发热出疹病例的血清学和病毒学检测

发布时间：2018-05-19 04:27

本文选题：含麻疹成分疫苗 + 发热出疹病例　；参考：《中国疫苗和免疫》2017年03期

【摘要】：目的分析接种含麻疹成分疫苗(Measles containing vaccine,MCV)后发热出疹病例(Rash and fever illness,RFI)的麻疹血清学和病毒学检测特征。方法采用酶联免疫吸附试验(ELISA)检测接种MCV后RFI的血清麻疹IgM抗体;用逆转录-聚合酶链反应(Reverse transcript polymerase chain reaction,RT-PCR)对麻疹病毒RNA样本进行检测,扩增麻疹病毒核蛋白(Nucleoprotein,N)基因羧基末端450个核苷酸片段;对扩增产物进行核苷酸序列测定和分析。结果 2010-2013年广东省共收检723例RFI的病原学标本,其中18例为接种MCV后RFI。在18例RFI中17例采集了血清标本,麻疹IgM抗体均阳性;18例病例的病原学样本经过序列测定分析,均属于A基因型,与中国S191疫苗株的核苷酸同源性为99.7%-100%。结论 MCV接种后RFI主要为麻疹疫苗相关病例;需加强RTPCR和核酸测序方法对病例的快速检测。
[Abstract]:Objective to analyze the characteristics of measles serological and virological detection in patients with fever and rash after inoculation with measles vaccine measures containing virus. Methods the serum measles IgM antibody of RFI inoculated with MCV was detected by Elisa, and the RNA samples of measles virus were detected by reverse transcript polymerase chain reaction (reverse transcript polymerase chain reaction). To amplify 450 nucleotide fragments at the carboxyl end of nucleoprotein N gene of measles virus, the nucleotide sequence of the amplified product was determined and analyzed. Results from 2010 to 2013, 723 RFI samples were collected from Guangdong Province, 18 of them were RFI after MCV inoculation. Serum samples were collected from 17 out of 18 cases of RFI. The etiological samples of 18 cases with positive measles IgM antibody were sequenced and all belonged to genotype A. The nucleotide homology with Chinese S191 vaccine strain was 99.7-100. Conclusion RFI after MCV inoculation is mainly measles vaccine-related cases, RTPCR and nucleic acid sequencing methods should be strengthened to detect the cases quickly.
【作者单位】：广东省疾病预防控制中心;中国疾病预防控制中心病毒病预防控制所;
【基金】：广东省医学科研基金指令性课题项目(项目编号:C2014003):应用多重实时荧光PCR技术快速鉴别麻疹疫苗病毒、野病毒、麻疹病毒H1基因型和风疹病毒;广东省医学科研基金项目(项目编号:A2009073)广东省麻疹N和H基因序列库的建立和应用研究
【分类号】：R511.1

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