HBV宫内感染胎盘组织TLR4及HBD-2的表达

发布时间：2018-05-19 13:46

  本文选题：HBV + 宫内感染　； 参考：《广西医科大学》2013年硕士论文

【摘要】：目的：研究乙型肝炎病毒宫内感染胎盘组织TLR4、HBD-2的表达。 方法：选择2011年6月到2012年6月广西医科大学第一附属医院分娩的乙肝标志物HBsAg、HBeAg为阳性且肝功能正常的孕妇46例为试验组,分娩后根据新生儿脐血清乙肝两对半及HBVDNA结果,分为宫内感染组11例,宫内未感染组35例。乙肝标志物均为阴性的正常孕妇20例为对照组,采用免疫组织化学SP法检测胎盘组织TLR4及HBD-2的表达。 结果：(1)孕妇血清HBsAg、HBeAg阳性的宫内感染率为23.9%。(2)免疫组化显示HBsAg、HBeAg阳性孕妇及正常对照组的胎盘组织中均表达TLR4、HBD-2; HBsAg、HBeAg阳性孕妇的胎盘组织TLR4、HBD-2的表达均低于正常对照组；宫内感染组TLR4、HBD-2的表达均低于宫内未感染组及正常对照组(P0.05)。(3)TLR4阳性信号位于胎盘各层细胞上,包括滋养层细胞(TC)、蜕膜细胞(DC)、胎盘绒毛间质细胞(VMC)、绒毛毛细血管内皮细胞(VCMC),以胞浆分布为主。HBD-2阳性信号主要分布在胎盘滋养层细胞(TC),以胞浆分布为主。 结论：(1)HBsAg、HBeAg阳性孕妇胎盘组织TLR4、HBD-2表达均低于正常对照组(P0.05)。(2)宫内感染组胎盘组织的TLR4、HBD-2的表达均低于HBV宫内未感染组及正常对照组(P0.05)。TLR4、HBD-2可能参与HBV宫内感染的垂直调控,是孕妇宫内感染HBV的保护因素。
[Abstract]:Objective: to study the expression of TLR4 and HBD-2 in placenta of intrauterine hepatitis B virus infection. Methods: from June 2011 to June 2012, 46 pregnant women with positive HBsAg HBeAg and normal liver function were selected as the test group. According to the results of umbilical cord blood serum hepatitis B and HBVDNA, 46 pregnant women who were delivered in the first affiliated Hospital of Guangxi Medical University from June 2011 to June 2012 were selected as the test group. The patients were divided into intrauterine infection group (n = 11) and intrauterine infection group (n = 35). The expression of TLR4 and HBD-2 in placenta was detected by immunohistochemical SP method in 20 normal pregnant women with negative HBV markers. Results the intrauterine infection rate of HBsAg HBeAg positive in pregnant women was 23.90.The expression of TLR4HBD-2in HBsAg HBeAg positive pregnant women and normal control group was detected by immunohistochemistry, and the expression of TLR4HBD-2 in HBsAg HBeAg positive pregnant women was lower than that in normal controls. The expression of TLR4nHBD-2 in intrauterine infection group was lower than that in uninfected intrauterine group and normal control group. The positive signal of TLR4 was located on the cells of placenta. These include trophoblastic cells, decidual cells, placental chorionic stromal cells and chorionic capillary endothelial cells. The cytoplasmic and cytoplasmic distribution of HBD-2 positive signals were mainly observed in trophoblastic cells of the placenta and mainly in the cytoplasm of the trophoblastic cells. Conclusion the expression of TLR4HBeAg HBD-2 in placenta of pregnant women with positive HBeAg is lower than that in normal control group (P 0.05). The expression of TLR4 HBeAg in placenta of intrauterine infection group is lower than that of HBV group and normal control group. It may be involved in the vertical regulation of intrauterine HBV infection, and the expression of TLR4HBD-2 may be involved in the vertical regulation of intrauterine HBV infection. It is a protective factor for intrauterine infection of HBV in pregnant women.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R512.62

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