固相金纳米棒光学免疫传感器对血吸虫病早期诊断价值的研究

发布时间：2018-05-20 13:42

本文选题：血吸虫 + 日本　；参考：《中国病原生物学杂志》2015年10期

【摘要】：目的评价固相金纳米棒光学免疫传感器对日本血吸虫病的早期诊断价值。方法利用构建的固相金纳米棒光学免疫传感器检测感染日本血吸虫1、2、4、6、8周的兔血清抗原,并与IHA抗体检测结果作比较。结果血吸虫感染第1周的兔血清IHA特异抗体检测为阴性,固相金纳米棒光学免疫传感器检测抗原呈阳性。2种方法检测血吸虫感染第2周、第4周、第6周和第8周的兔血清均为阳性。结论固相金纳米棒光学免疫传感器对日本血吸虫感染兔血清抗原具有早期识别能力,感染第1周的兔血清抗原检测即呈阳性反应。该方法可用于日本血吸虫病的早期诊断,也可为其他寄生虫病的早期诊断带来新的尝试。
[Abstract]:Objective to evaluate the value of solid-phase gold nanorods optical immunosensor in early diagnosis of schistosomiasis japonica. Methods A solid phase gold nanorods optical immunosensor was used to detect the serum antigens of rabbits infected with Schistosoma japonicum for 8 weeks, and the results were compared with the results of IHA antibody detection. Results in the first week of schistosomiasis infection, the serum IHA specific antibody was negative in rabbits, and the antigen was detected by optical immunosensor with solid phase gold nanorods at week 2 and week 4. At week 6 and week 8, the rabbit serum was positive. Conclusion Solid-phase gold nanorods optical immunosensor has the ability of early recognition of serum antigen of rabbits infected with Schistosoma japonicum, and the detection of serum antigen in the first week of infection is positive. This method can be used for the early diagnosis of schistosomiasis japonicum, but also for the early diagnosis of other parasitic diseases.
【作者单位】：中南大学基础医学院医学寄生虫学系;湖南大学化学化工学院化学生物传感器与计量学国家重点实验室;
【基金】：国家自然科学基金项目(No.81271862) 湖南省创新平台与人才计划(应用基础研究)重点项目(No.2015JC3022) 中南大学中央高校基础研究业务费专项资金项目(No.2012zzts028)
【分类号】：R532.21

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