色氨酸缺乏及外源性吲哚对沙眼衣原体生长的影响

发布时间：2018-05-20 19:48

本文选题：沙眼衣原体 + Hela细胞　；参考：《中国皮肤性病学杂志》2017年01期

【摘要】：目的探讨色氨酸缺乏及外源性吲哚对沙眼衣原体E-UW-5/Cx和D-UW-5/Cx型标准株及临床株生长的影响。方法分别用DMEM-10细胞培养液(简称:DMEM-10)、缺乏色氨酸的DMEM-10细胞培养液[简称:DMEM-10(-Trp)]和缺乏色氨酸的DMEM-10细胞培养液中加入10μM外源性吲哚的细胞培养液[简称:DMEM-10(-Trp)+IND]三种液体培养沙眼衣原体至48h,甲醇固定,计数包涵体,观察色氨酸缺乏及外源性吲哚对E型和D型沙眼衣原体标准株及临床株生长的影响。结果 DMEM-10组(-Trp)组衣原体包涵体计数明显低于DMEM-10和DMEM-10(-Trp)+IND组,且差异有统计学意义(P0.01);但DMEM-10组和DMEM-10(-Trp)+IND组包涵体计数差异无统计学意义(P0.05);E型标准株、临床株与D型标准株、临床株之间生长差异也无统计学意义(P0.05)。结论色氨酸缺乏不利于沙眼衣原体的生长,补充外源性吲哚后,沙眼衣原体可恢复其感染活力。
[Abstract]:Objective to investigate the effects of tryptophan deficiency and exogenous indole on the growth of Chlamydia trachomatis E-UW-5/Cx and D-UW-5/Cx standard strains and clinical strains. Methods the DMEM-10 cell culture solution (DMEM-10), DMEM-10 cell culture solution for tryptophan (abbreviated as DMEM-10 (-Trp)) and DMEM-10 cells lacking tryptophan were added to 10 micron respectively. The cell culture solution of exogenous indole (DMEM-10 (-Trp) +IND]) was used to cultivate Chlamydia trachomatis to 48h, methanol fixation and inclusion bodies. The effects of tryptophan deficiency and exogenous indole on the growth of Chlamydia trachomatis and clinical strains of E and D type trachomatis were observed. Results the number of Chlamydia inclusion bodies in group DMEM-10 (-Trp) was significantly lower than DMEM The difference between -10 and DMEM-10 (-Trp) +IND group was statistically significant (P0.01), but there was no statistical difference between DMEM-10 and DMEM-10 (-Trp) +IND group, and there was no significant difference in the growth difference between clinical strain and D type standard strain. Conclusion tryptophan deficiency was not beneficial to the growth of Chlamydia trachomatis. After being loaded with exogenous indole, Chlamydia trachomatis can restore its infection activity.
【作者单位】：泰山医学院附属医院皮肤科;天津医科大学总医院皮肤科;
【基金】：山东省自然科学基金项目(ZR2011HL009)
【分类号】：R691.3

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