自助采集尿液及干血斑样本检测HIV的结果评价

发布时间：2018-05-21 14:19

本文选题：自助采样包 + 艾滋病病毒　；参考：《中国艾滋病性病》2017年05期

【摘要】：目的通过对一种艾滋病病毒(HIV)自助采样包采集的样本进行检测,探讨其临床应用的可行性。方法针对已知感染状态的男男性行为者(MSM)、静脉吸毒者(IDUs)和健康体检人群,共807人;未知感染状态的暗娼283人;由专业人员分发并回收HIV的自助采样包。利用酶联免疫吸附试验对收集的尿液及干血斑样本进行HIV抗体检测,并对暗娼进行干血斑核酸检测,分析不同方法检测结果的一致性。结果尿液样本抗体检测结果与已知感染状态比较,健康体检人群、MSM、IDUs以及总人群的检测一致率分别为96.55%、98.99%、95.20%、97.03%;干血斑样本抗体检测结果与已知感染状态比较,健康体检人群、MSM、IDUs以及总人群的检测一致率分别为98.47%、100%、98.80%、99.13%。对未知感染状态的暗娼,将抗体检测结果与干血斑核酸检测结果进行比较,干血斑样本与尿液样本的一致率分别为96.82%、97.88%;将干血斑与尿液样本的抗体检测结果比较,检测一致率为96.82%。结论对采用自助采样包采集的样本进行检测,发现具有较高的检测效能,说明自助采样包采集的样本,不仅适用于高危人群,在自然人群中也有实用价值。
[Abstract]:Objective to investigate the clinical feasibility of a self-sampling kit of HIV-positive virus (HIV). Methods A total of 807 men who had sex with men with known infection status (IDUs) and healthy people were enrolled in the study. 283 female sex workers with unknown infection status were included in the study. The self-sampling kits of HIV were distributed and recovered by professionals. Enzyme linked immunosorbent assay (Elisa) was used to detect HIV antibody in urine and dry blood spot samples, and to detect nucleic acid in dry blood spot of female sex workers, and to analyze the consistency of the results of different methods. Results the results of urine antibody detection were compared with those of known infection status. The consistent detection rates of MSMIDUs and the total population in healthy people were 96.55 and 98.9999 and 95.2097.03, respectively, and the results of antibody detection in dry blood spot samples were compared with those of known infection status. The consistency rates of MSMN IDUs and the total population were 98.470.100 and 98.800.99.13, respectively. The results of antibody detection were compared with the results of nucleic acid detection in dry blood spots. The consistent rates of dry blood spot samples and urine samples were 96.82 ~ 97.88, and the results of antibody detection of dry blood spots and urine samples were 96.82 ~ (2) and 96.82% respectively. Conclusion it is found that the sample collected by self-help sampling bag has higher detection efficiency, which indicates that the sample collected by self-help sampling bag is not only suitable for high risk population, but also has practical value in natural population.
【作者单位】：中国疾病预防控制中心性病艾滋病预防控制中心;云南省德宏州疾病预防控制中心;云南省疾病预防控制中心;四川省凉山州疾病预防控制中心;
【基金】：中美艾滋病项目(GAP)~~
【分类号】：R512.91

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