维生素D通过调控NO水平抑制结核杆菌诱导的巨噬细胞凋亡

发布时间：2018-05-24 00:50

本文选题：维生素D + 结核杆菌　；参考：《免疫学杂志》2015年03期

【摘要】：目的探索维生素D对结核杆菌Mr19 000脂蛋白诱导的人类巨噬细胞损伤的作用。方法首先从健康志愿者血样中提取人巨噬细胞,同时从结核杆菌中制备Mr19 000脂蛋白。MTT法检测了Mr19 000脂蛋白以及1,25(OH)2D3对巨噬细胞增殖的作用,流式细胞术则用来评价二者对巨噬细胞凋亡的影响。接下来,我们通过MTT、FCM和ELISA等方法深入探究了维生素D对Mr19 000脂蛋白诱导的巨噬细胞损伤的作用及其机制。结果 16μg/ml Mr19 000脂蛋白作用巨噬细胞24 h后可显著减低巨噬细胞增殖并诱导巨噬细胞凋亡。相反,维生素D对人类正常巨噬细胞几乎没有毒性作用,能够阻断Mr19 000脂蛋白对巨噬细胞的损害。除此之外,维生素D还明显降低了Mr19 000脂蛋白诱导的NO/i NOS水平。结论维生素D可通过调控NO/i NOS水平阻碍结核杆菌毒力因子Mr19 000脂蛋白诱导的人类巨噬细胞凋亡。
[Abstract]:Objective to investigate the effect of vitamin D on human macrophage injury induced by Mr19 000 lipoprotein of Mycobacterium tuberculosis. Methods Human macrophages were extracted from the blood samples of healthy volunteers, and Mr19 000 lipoprotein was prepared from Mycobacterium tuberculosis. The effects of Mr19 000 lipoprotein and OHH 2D3 on the proliferation of macrophages were measured. Flow cytometry was used to evaluate the effects of both on macrophage apoptosis. Then we investigated the effects of vitamin D on macrophage injury induced by Mr19 000 lipoprotein and its mechanism by means of MTT FCM and ELISA. Results 16 渭 g/ml Mr19 000 lipoprotein could significantly reduce macrophage proliferation and induce macrophage apoptosis after 24 h treatment. In contrast, vitamin D has little toxic effect on normal human macrophages and blocks Mr19 000 lipoprotein damage to macrophages. In addition, vitamin D also significantly reduced the level of NO/i NOS induced by Mr19 000 lipoprotein. Conclusion Vitamin D can inhibit the apoptosis of human macrophages induced by the virulence factor Mr19 000 lipoprotein of Mycobacterium tuberculosis by regulating the level of NO/i NOS.
【作者单位】：第四军医大学西京医院呼吸科;
【分类号】：R52

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