IL-2、IL-4、IL-6、IFN-γ、IL-17A在肝癌组织中表达及其与乙肝病毒感染的关系

发布时间：2018-05-24 08:04

本文选题：肝癌 + Th　；参考：《中国免疫学杂志》2015年04期

【摘要】：目的:研究Th17(IL-17A)在肝细胞癌发生发展中的作用,尤其与乙肝病毒感染的关系。方法:收集39例原发性肝细胞癌患者的癌与非癌组织,应用流式细胞微球阵列术(CBA)检测IL-2、IL-4、IL-6、IFN-γ、IL-17A细胞因子水平,并与患者临床资料进行统计比较分析。结果:肝癌组织中IL-2、IL-4、IFN-γ表达水平好[分别为(4.61±0.28)、(3.37±0.58)、(3.08±1.08)pg/ml]显著低于非癌组织[分别为(5.57±0.59)、(3.77±0.70)、(3.69±1.20)pg/ml],而IL-6、IL-17A水平[分别为(280.09±254.68)、(2.66±1.66)pg/ml]显著高于非癌组织[分别为(6.58±1.92)、(1.49±0.98)pg/ml],同时无论癌组织还是非癌组织,高乙肝病毒载量(1 000 U/ml)的组织IL-17A的表达[(3.45±1.86)pg/ml]显著高于低病毒载量(1 000U/ml)的组织[(1.97±1.16)pg/ml]。结论:Th17细胞分泌的细胞因子IL-17A在肝癌组织中高表达,IL-2、IL-4和IFN-γ可能抑制其表达,而IL-6可能促进其表达;乙肝病毒感染有可能促进Th17的表达,从而降低患者的预后。
[Abstract]:Aim: to study the role of Th17 IL-17A in the occurrence and development of hepatocellular carcinoma, especially its relationship with hepatitis B virus infection. Methods: tumor and non-cancer tissues were collected from 39 patients with primary hepatocellular carcinoma (HCC). The levels of IL-2hIL-4- 纬 IL-17A cytokines were detected by flow cytometry microsphere array (CBA) and compared with the clinical data of the patients. Results: the expression level of IL-2hIL-4 and IFN- 纬 in HCC was significantly lower than that in non-cancerous tissues (3.37 卤0.58 卤3.08 卤1.08)pg/ml, 4.61 卤0.28, respectively), and the level of IL-6IL-17A [280.09 卤254.68 卤2.66 卤1.66)pg/ml] was significantly higher than that in non-cancerous tissues (6.58 卤1.922 卤1.49 卤0.98)pg/ml, respectively), and the level of IL-6IL-17A in non-cancerous tissues was significantly higher than that in non-cancerous tissues (5.57 卤0.59, 3.77 卤0.70 卤3.69 卤1.20)pg/ml, respectively), and the level of IL-6IL-17A in HCC was significantly higher than that in non-cancerous tissues [6.58 卤1.922.49 卤0.98)pg/ml, respectively], and the expression of IL-6IL-17A was significantly higher than that in non-cancerous tissues. The expression of IL-17A [3.45 卤1.86)pg/ml] in tissues with high HBV load (1 000 U / ml) was significantly higher than that with low viral load (1 000 U / ml) [1.97 卤1.16)pg/ml]. Conclusion the overexpression of IL-2IL-4 and IFN- 纬 in hepatocellular carcinoma may be inhibited by the cytokine IL-17A secreted by Th17 cells, while the expression of IL-6 may be enhanced by IL-6, and hepatitis B virus infection may promote the expression of Th17 so as to decrease the prognosis of the patients.
【作者单位】：福建省肿瘤医院肿瘤免疫学研究室福建医科大学教学医院福建省肿瘤转化医学重点实验室;
【基金】：福建省卫生厅青年科研课题基金项目(2011-2-17)
【分类号】：R735.7;R512.62

【参考文献】