Th22细胞、白细胞介素-22在乙型肝炎病毒相关原发性肝癌中的表达及意义

发布时间：2018-05-26 21:53

本文选题：原发性肝癌 + 乙型肝炎病毒　；参考：《广东医学》2017年13期

【摘要】：目的探讨乙型肝炎病毒(HBV)相关原发性肝癌(HCC)患者Th22细胞以及特征性细胞因子白细胞介素(IL)-22表达水平的变化。方法选择HBV-HCC患者(HCC组)32例,HBV-肝硬化(LC)患者(LC组)24例作为研究对象,采用流式细胞检测方法检测外周血Th22细胞的表达频数;同时选择15例健康者作为对照组。选择外科手术切除HBV-HCC、癌旁LC组织标本24份,采用免疫组化方法检测肝组织中IL-22的表达情况。结果 HCC组患者外周血Th22细胞表达频数为(6.95±2.73)%;LC组患者为(4.80±2.01)%;对照组为(1.74±0.94)%,3组间相比,差异有统计学意义(F=28.434,P=0.000);HCC组高于LC组和对照组(P=0.001、0.000);LC组患者高于对照组(P=0.000)。HBV-HCC组织中IL-22表达阳性细胞数为(110.2±32.4)个/高倍视野;癌旁LC组织中为(61.3±22.4)个/高倍视野,差异有统计学意义(t=6.082,P=0.000)。结论Th22细胞及特征性细胞因子IL-22在HBV-HCC发生、发展过程中起到一定的作用。
[Abstract]:Objective to investigate the expression of Th22 cells and interleukin IL-22 in patients with hepatocellular carcinoma (HCC) associated with hepatitis B virus (HBV). Methods the frequency of Th22 expression in peripheral blood was detected by flow cytometry (FCM) in 32 patients with HBV-HCC and 15 healthy controls. The expression of IL-22 in liver tissues was detected by immunohistochemical method in 24 specimens of adjacent LC tissues after surgical resection of HBV-HCC. Results the frequency of expression of Th22 cells in peripheral blood of HCC group was 6.95 卤2.73 and that of LC group was 4.80 卤2.01g, while that of control group was 1.74 卤0.94%. The difference was statistically significant (P < 0.05). The number of IL-22 positive cells in HCC group was higher than that in LC group and control group. The number of IL-22 positive cells in HCC tissue was 110.2 卤32.4 / high field of vision and 61.3 卤22.4 / high field of vision in adjacent LC tissue, and the difference was statistically significant. Conclusion Th22 cells and characteristic cytokine IL-22 play a role in the pathogenesis and development of HBV-HCC.
【作者单位】：南通市第三人民医院感染科;南通市第三人民医院检验科;南通市第三人民医院病理科;
【基金】：南通市社会事业科技创新与示范项目(编号:HS2014081)
【分类号】：R512.62;R735.7

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