云南泸沽湖、程海流域广州管圆线虫病流行病学研究及其地理信息系统的应用探讨

发布时间：2018-05-30 07:25

本文选题：广州管圆线虫 + 程海　；参考：《大理学院》2014年硕士论文

【摘要】：【目的】云南泸沽湖、程海流域进行广州管圆线虫流行病学调查，初步掌握云南泸沽湖、程海流域广州管圆线虫中间宿主和终宿主的分布及感染情况，检测该流域内人群广州管圆线虫感染情况；结合地理信息系统建立云南泸沽湖、程海流域广州管圆线虫流行病学调查数据库，分析影响广州管圆线虫流行及分布的因素。【方法】广州管圆线虫中间宿主螺蛳和蛞蝓采用胃蛋白酶消化法检查其感染状况；终宿主鼠采用剖检法和免疫酶联法检查其感染状况；GPS定位仪实地定位中间宿主和终宿主的调查点的经纬度和海拔；云南泸沽湖、程海流域人群作流行病学问卷调查，免疫酶联法检测被抽取人群血清的广州管圆线虫特异性Ig抗体；用Spss19.0对云南泸沽湖、程海流域广州管圆线虫流行病学调查数据进行统计分析；结合地理信息系统建立云南泸沽湖、程海流域广州管圆线虫流行病学调查数据库；基于地理信息系统分析影响广州管圆线虫流行及分布的因素。【结果】 1.捕获广州管圆线虫中间宿主4种螺蛳和1种蛞蝓，共3615只，经检测均未发现广州管圆线虫。泸沽湖流域采集到3种螺蛳和1种蛞蝓，分别是：中华圆田螺、铜锈环棱螺、琵琶萝卜螺和复套蛞蝓；程海流域采集到4种螺蛳和1种蛞蝓，分别是：中华圆田螺、铜锈环棱螺、琵琶萝卜螺、钉螺和复套蛞蝓。 2.捕获广州管圆线虫终宿主3种鼠，共234只，剖检法均未发现广州管圆线虫；79份鼠血清酶联免疫法检测广州管圆线虫特异性IgG抗体,结果均为阴性。 3.采集云南泸沽湖、程海流域居民血清548份，免疫酶联法检测广州管圆线虫特异性IgG抗体，共26份阳性血清，阳性率为4.74%。少数民族人群血清阳性率较高;有野外喝生水习惯的居民血清阳性率较高。 4.结合地理信息系统建立了云南泸沽湖、程海流域广州管圆线虫流行病学调查快速查询数据库，并将调查数据进行了可视化描述。 5.云南泸沽湖、程海流域距水域不同距离缓冲区内居民血清广州管圆线虫特异性IgG抗体阳性率差异无统计学意义；居民血清中广州管圆线虫特异性IgG抗体OD值空间分布不具有显著的空间自相关性；在宁蒗县与程海县交界处存在广州管圆线虫特异性IgG抗体OD值高值的聚集区域，为该地区广州管圆线虫的防治提供了依据。 6.基于温度分析结果显示泸沽湖、程海流域所在的宁蒗县、永胜县不适合广州管圆线虫的生长发育。【结论】 1.云南泸沽湖、程海流域均有广州管圆线虫传播宿主的存在，但没有发现广州管圆线虫虫体，该区域的二月平均温度较低，不适合广州管圆线虫的生长发育，云南泸沽湖、程海流域暂不能视为广州管圆线虫自然疫源地。 2.云南泸沽湖、程海流域居民血清标本进行广州管圆线虫特异性IgG抗体阳检测发现26例阳性标本。其中，根据调查问卷统计显示，少数民族居民血清阳性率高于汉族居民血清阳性率，，有野外喝生水习惯的居民血清阳性率高于没有在野外喝生水习惯的居民。 3.利用地理信息系统对本次云南泸沽湖、程海流域广州管圆线虫流行病学调查数据进行了可视化描述并将数据结合图像建立了泸沽湖、程海流域广州管圆线虫流行病学信息的快速查询系统。 4.本次检测云南泸沽湖、程海流域居民血清广州管圆线虫特异性IgG抗体，发现其学清的OD值在整体上没有空间自相关性，但在宁蒗县与永胜县的东南交界处附近可能存在血清OD值高值的聚集区域，该地区广州管圆线虫的防治工作不能松懈。
[Abstract]:[Objective]
Yunnan Lugu Lake and Cheng Hai Basin carried out an epidemiological survey of Guangzhou tube nematode, preliminarily grasps the distribution and infection of the Yunnan Lugu Lake, the middle host and the final host of the Guangzhou tube nematode in the Cheng Hai Valley, and detected the infection of the Guangzhou tube nematode in the river basin, and established the Yunnan Lugu Lake and the Guangzhou of the Cheng Hai Basin in combination with the geographic information system. The epidemiology and distribution of Guangzhou's roundworm were analyzed by the database of epidemiology.
[method]
The infection status of the snail and slug in the middle host of Guangzhou nematode was examined by pepsin digestion; the infection status of the final host mice was examined by caesarean section and immunoenzyme linked assay; the latitude and latitude of the investigation points of the intermediate host and the final host by GPS locator on the spot; the population of the Lugu Lake in Yunnan and the area of the Cheng Hai River Basin for epidemiology. A questionnaire survey was conducted to detect the specific Ig antibody of Guangzhou tube nematode in the serum of the extracted population, and the epidemiological survey data of Guangzhou tube nematode in Yunnan Lugu Lake and Cheng Hai Basin were statistically analyzed with Spss19.0. The epidemiological investigation of Guangzhou tube nematode in the Yunnan Lugu Lake and the Cheng Hai River Basin were established by combining the geographic information system. The geographic information system (GIS) was used to analyze the factors affecting the prevalence and distribution of Guangzhou C. elegans.
[results]
1. a total of 4 species of snail and 1 slugs in the middle host of Guangzhou nematode were captured, and 3615 were found. There were 3 species of snails and 1 slugs in the Lugu Lake basin. They were Chinese snail, rounded spiral snail, lute, and multiple slugs, and 4 species of snail and 1 slugs were collected in the Cheng Hai basin, respectively: Hua Yuan snail, rust rust, snails, lute, snails, snails and compound slugs.
2. a total of 3 mice were captured in the final host of Guangzhou tube nematode, and there were 234 rats. No Guangzhou tube nematode was found in the caesarean section. 79 mice of serum enzyme linked immunosorbent assay (ELISA) were used to detect the specific IgG antibody of the Guangzhou tube nematode. The results were all negative.
3. in Yunnan Lugu Lake, 548 sera were collected from the residents of the Cheng Hai River Basin, and the immunoenzyme linked immunosorbent assay was used to detect the specific IgG antibody of the Guangzhou tube nematode, with a total of 26 positive sera. The positive rate of the positive rate was higher in the 4.74%. minority group, and the positive rate of the residents with the habit of drinking water in the field was higher.
4. combined with geographic information system, Yunnan Lugu Lake, Guangzhou River Valley nematode epidemiological investigation database was established, and the survey data were visualized.
5. Yunnan Lugu Lake, there was no significant difference in the positive rate of sera specific IgG antibody of Guangzhou tube nematode, and there was no significant spatial autocorrelation in the spatial distribution of the specific IgG antibody of Guangzhou tube nematode in the residents' sera, and there was Guangzhou at the junction of the county of Ning Lang County and Cheng Hai county. The aggregation area of the high OD value of the specific IgG antibody of C.elegans provides a basis for the prevention and treatment of the Guangzhou roundworm.
6. based on the results of temperature analysis, it is shown that the Yongsheng Lugu Lake is not suitable for the growth and development of the Guangzhou roundworm.
[Conclusion]
1. Yunnan Lugu Lake, Cheng Hai Basin all the host of Guangzhou tube nematode transmission, but not the Guangzhou tube round line worm body, the area of the average temperature in February is low, it is not suitable for the growth and development of Guangzhou tube nematode, Yunnan Lugu Lake, the Cheng Hai Basin can not be considered as the natural foci of the Guangzhou tube nematode.
2. Yunnan Lugu Lake, the serum samples of the residents in the Cheng Hai River Basin were tested for 26 positive specimens of the specific IgG antibody positive of Guangzhou tube nematode. Among them, the positive rate of the serum positive rate of the ethnic minority residents was higher than that of the Han population, and the positive rate of the people who had the habit of drinking water in the field was higher than that in the field. A inhabitant of the habit of drinking water.
3. the geographical information system was used to visualize the epidemiological survey data of Guangzhou tube nematode in Yunnan Lugu Lake and Cheng Hai River Basin, and to set up a rapid inquiry system for the epidemiological information of the Guangzhou tube nematode in the Lugu Lake and the Cheng Hai Basin by combining the data with the data.
4. in Yunnan Lugu Lake, the residents of the Cheng Hai River Basin were found to have a serum specific IgG antibody of Guangzhou tube nematode. It was found that there was no spatial autocorrelation on the whole, but there might be a high concentration of serum o value in the vicinity of the southeastern border of Yongsheng county. The prevention and treatment of Guangzhou tube nematode in this area could not be loose. Lax.
【学位授予单位】：大理学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R532.1

【参考文献】