抗菌肽CGA-N12对腹腔注射热带念珠菌小鼠的治疗作用

发布时间：2018-05-30 23:16

本文选题：抗菌肽 + CGA-N　；参考：《动物医学进展》2017年04期

【摘要】：CGA-N12为嗜铬粒蛋白A的N端65-76位氨基酸组成的衍生肽,前期研究表明,CGA-N12具有特异性抗念珠菌活性,尤其对临床病原真菌热带念珠菌拮抗活性最强。为研究CGA-N12体内抗真菌活性,建立了热带念珠菌深部感染小鼠模型,分别用每日15mg/kg和30mg/kg的CGA-N12对小鼠进行治疗,并设每日15mg/kg伊曲康唑治疗对照组和感染对照组。通过测定治疗后小鼠存活率、体重变化、肾脏载菌量,以及组织器官病理变化,判断CGA-N12对小鼠的治疗效果。结果表明,每日15 mg/kg和30mg/kg的CGA-N12治疗14d后与感染对照组相比,小鼠肾脏载菌量分别降低97.6%和99.1%,小鼠存活率分别提高40%和45%,体重分别增加1.3g和5.9g,免疫器官指数均显著增加,而与治疗对照组差异不显著。病理切片观察,每日15mg/kg和30mg/kg的CGA-N12对组织器官的病变均有明显改善,并且没有出现伊曲康唑治疗后产生的肝小叶血管内皮细胞增生和肾出血现象。结果表明,CGA-N12对热带念珠菌深部感染小鼠具有治疗作用。
[Abstract]:CGA-N12 is a derivative peptide of N-terminal 65-76 amino acids of chromogranin A. Previous studies have shown that CGA-N12 has specific activity against Candida candida, especially the most antagonistic activity against clinical pathogenic fungi Candida tropicalis. In order to study the antifungal activity of CGA-N12 in vivo, a mouse model of deep infection of Candida tropicalis was established. The mice were treated with daily CGA-N12 of 15mg/kg and 30mg/kg, respectively. The mice were treated with 15mg/kg itraconazole daily as control group and as infected control group. The therapeutic effect of CGA-N12 on mice was evaluated by measuring the survival rate, weight change, kidney bacterial load and pathological changes of tissues and organs after treatment. The results showed that compared with the control group, 15 mg/kg and 30mg/kg CGA-N12 for 14 days decreased the carrying amount of bacteria in kidney by 97.6% and 99.1, the survival rate of mice increased by 40% and 45%, the weight of mice increased by 1.3 g and 5.9 g, and the immune organ index increased significantly. However, there was no significant difference between the control group and the treatment group. The pathological sections showed that both CGA-N12 of 15mg/kg and 30mg/kg could significantly improve the pathological changes of tissues and organs, and there was no proliferation of endothelial cells and renal hemorrhage in hepatic lobules after itraconazole treatment. The results showed that CGA-N 12 had therapeutic effect on deep infection of Candida tropicalis in mice.
【作者单位】：河南工业大学生物工程学院;
【基金】：国家自然科学基金项目(31071922,31572264) 河南省省属高校基本科研业务费专项资金项目(2015RCJH03)
【分类号】：R519.3

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