成纤维细胞激活蛋白与慢性乙型肝炎相关性肝病的关系

发布时间：2018-06-05 01:55

本文选题：肝炎病毒 + 乙型　；参考：《广东医学》2017年06期

【摘要】：目的通过比较乙型肝炎(乙肝)相关性肝癌、乙肝肝硬化以及慢性乙肝患者肝组织中成纤维细胞激活蛋白(FAP)水平,探讨FAP在乙肝相关性慢性肝脏疾病的表达差异及其意义。方法收集47例乙肝相关性肝癌、29例乙肝肝硬化(S4期)、22例慢性乙肝(S1~S3期)及17例因良性病变行肝切除术的肝组织,采用反转录聚合酶链反应(RT-PCR)分别检测其中FAP的表达情况;采用酶联免疫吸附(ELISA)方法检测组织及血清中FAP蛋白表达水平。结果在肝癌、肝硬化、肝炎以及对照组肝组织中,FAP mRNA表达的差异倍数值分别是4.73±1.60、1.86±1.04、0.98±0.80及1.00±0.00,四者比较差异有统计学意义(F=7.156,P=0.000);ELISA结果显示FAP蛋白在肝癌患者组织中1μg总蛋白平均含量为(1 288.28±695.82)pg/mL,而肝硬化组织为(1 176.11±677.43)pg/mL,肝炎组织为(1 044.58±389.48)pg/mL,对照组为(848.70±540.74)pg/mL,差异有统计学意义(F=3.004,P=0.034)。结论 FAP在肝炎、肝硬化、肝癌以及对照组的mRNA及蛋白表达水平存在差异,随着疾病的进展,FAP呈渐进性表达。
[Abstract]:Objective to compare the levels of fibroblast activator protein (FAP) in liver tissues of patients with hepatitis B (HBV) associated liver cancer, hepatitis B cirrhosis and chronic hepatitis B, and to explore the significance of FAP expression in hepatitis B related chronic liver diseases. Methods the expression of FAP was detected by reverse transcriptase polymerase chain reaction (RT-PCR) in the liver tissues of 47 patients with Hepatitis B associated liver cancer and 22 patients with chronic Hepatitis B S1 / S3 and 17 patients with benign liver diseases. The expression of FAP was detected by reverse transcriptase polymerase chain reaction (RT PCR). The expression of FAP was detected by reverse transcriptase polymerase chain reaction (RT PCR). Enzyme linked immunosorbent assay (Elisa) was used to detect the expression of FAP protein in tissues and serum. Results in liver cancer, liver cirrhosis, The difference multiple values of mRNA expression in liver tissues of hepatitis and control group were 4.73 卤1.60,1.86 卤1.04 卤0.98 卤0.80 and 1.00 卤0.000.The results of Elisa showed that the average content of 1 渭 g total protein of FAP protein in liver cancer was 1 288.28 卤695.82 渭 g / mL, while that in liver cirrhosis was 1.00 卤0.000.0.000. The results of Elisa showed that the average content of FAP protein in liver cancer was 1 288.28 卤695.82 渭 g 路mL ~ (-1), while that of liver cirrhosis was 1 渭 g 路mL ~ (-1). The tissues were 1 176.11 卤677.43 g / mL, 1 044.58 卤389.48 渭 g / mL in hepatitis tissue and 848.70 卤540.74 渭 g / mL in control group. The difference was statistically significant (P < 0.05). Conclusion there are differences in the expression of mRNA and protein in hepatitis, liver cirrhosis, liver cancer and control group, and the expression of mRNA is progressive with the progression of the disease.
【作者单位】：中山大学附属第三医院感染科;
【基金】：国家自然科学基金资助项目(编号:81500462)
【分类号】：R512.62;R575

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