绿原酸、异绿原酸对烟曲霉菌生物被膜抑制作用的体外研究

发布时间：2018-06-08 00:02

  本文选题：烟曲霉菌 + 生物被膜　； 参考：《广西医科大学》2014年硕士论文

【摘要】：目的：通过在体外复制烟曲霉菌(Aspergillus fumigates)的生物被膜(Biofilm, BF)模型，研究绿原酸(Chlorogenic acid，CRA)、异绿原酸(Isochlorogenic acid，IRC)对生物被膜的影响。 方法：选取临床分离的烟曲霉菌构建体外生物被膜模型，微量液基稀释法测定最低抑菌浓度(Minimal inhibition concentration, MIC)，，将菌株分为空白对照组、实验组（CRA1024μg/ml、CRA512μg/ml、CRA256μg/ml、IRC1000μg/ml、IRC500μg/ml、IRC250μg/ml），扫描电子显微镜(Scanningelectron microscopy，SEM)、激光共聚焦扫描显微镜(Laser confocal scanningmicroscope， CLSM)定性观察BF；结晶紫染色法半定量生物被膜量。 结果：37℃培养24h烟曲霉菌形成早期BF，48h形成成熟期BF。实验组烟曲霉菌BF均较其相应空白对照组空洞、稀疏，但菌丝未见明显减少，死活菌数也无明显差别。CRA组早期结晶紫半定量结果分别为（1.05±0.19）、（1.14±0.26）、（1.16±0.14），IRC组为（1.18±0.06）、（1.21±0.06）、（1.26±0.04），均少于空白对照组（1.91±0.17），差异具有统计学意义（P0.05）；CRA组成熟期结晶紫半定量结果为（2.25±0.05）、（2.27±0.05）、（2.31±0.03），IRC组为（2.26±0.02）、（2.27±0.02）、（2.29±0.04），也均少于空白对照组（2.36±0.01），差异具有统计学意义（P0.05）。 结论：1、可以在体外建立烟曲霉菌生物被膜模型；2、CRA、IRC对体外烟曲霉菌BF形成有抑制作用。
[Abstract]:Objective: to study the effect of Lv Yuan acid Chlorogenic acidoglycan (Isochlorogenic acidogenic) on biofilm by replicating the biofilm model of Aspergillus fumigatesin vitro. Methods: a biofilm model was established in vitro by clinical isolates of Aspergillus fumigates.Methods: the biofilm model of Aspergillus fumigatesin vitro was established. Minimal inhibition concentration was determined by microdilution method. The strains were divided into blank control group. CRA1024 渭 g / ml CRA512 渭 g / ml CRA256 渭 g / ml IRC1000 渭 g / ml IRC500 渭 g / ml IRC250 渭 g / ml, scanning electron microscope (SEM), laser confocal scanning microscope (laser confocal scanning microscope), laser confocal scanning microscope (laser confocal scanning microscope), laser scanning microscope (CLSM), and semi-quantitative biofilm quantity of CRA 256 渭 g / ml. The BF of Aspergillus fumigatus in the experimental group was less than that in the control group, but the mycelium did not decrease obviously. 姝绘椿鑿屾暟涔熸棤鏄庢樉宸�鍒�.CRA缁勬棭鏈熺粨鏅剁传鍗婂畾閲忕粨鏋滃垎鍒�涓�(1.05卤0.19),(1.14卤0.26),(1.16卤0.14),IRC缁勪负(1.18卤0.06),(1.21卤0.06),(1.26卤0.04),鍧囧皯浜庣┖鐧藉�圭収缁�(1.91卤0.17),宸�寮傚叿鏈夌粺璁″�︽剰涔

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