乳酸抑制精液来源淀粉样纤维的形成

发布时间：2018-06-09 06:08

本文选题：乳酸 + 淀粉样纤维　；参考：《南方医科大学学报》2017年07期

【摘要】：目的探究乳酸对精液来源的淀粉样纤维形成的抑制作用。方法 2 mg/m L的PAP248-286多肽与不同浓度乳酸(4、2、1、0.5、0.25、0.125 mg/m L)分别孵育,在不同时间点取样,用硫磺素T和刚果红染色法分别检测淀粉样纤维形成的过程;采用圆二色谱法检测乳酸对PAP248-286形成β-片层结构的影响;采用透射电子显微镜观察乳酸对淀粉样纤维形态的影响;用病毒感染实验,检测与乳酸孵育的不同时间点,精液淀粉样纤维促进病毒感染的能力;将2 mg/m L的PAP248-286多肽与不同浓度的健康女性阴道分泌液孵育后,用硫磺素T和刚果红染色法评价阴道分泌液抑制精液淀粉样纤维形成的作用。结果乳酸体外能浓度依赖性地抑制精液淀粉样纤维的形成,在48 h内,0.5 mg/m L乳酸能够抑制2 mg/m L的淀粉样纤维SEVI的形成;在48 h内,1 mg/m L的乳酸能抑制精液淀粉样纤维(2 mg/m L)的β-片层结构的形成,透射电子显微镜观察到1 mg/m L的乳酸能完全抑制2 mg/m L PAP248-286聚合形成淀粉样纤维;与乳酸孵育的多肽PAP248-286,失去了促进病毒感染的能力;阴道分泌液浓度依赖性地抑制SEVI的形成,48 h内,2 mg/m L的PAP248-286在67%阴道分泌液存在时几乎无SEVI生成。结论乳酸能抑制精液来源的淀粉样纤维的形成。
[Abstract]:Objective to investigate the inhibitory effect of lactic acid on amyloid formation in semen. Methods (2) the polypeptide PAP248-286 of mg/m L was incubated with 0.25 0.125 mg/m / L of different concentrations of PAP248-286. The process of amyloid formation was detected by sulfur T and Congo red staining at different time points. Circular dichroism was used to detect the effect of lactic acid on the formation of 尾-lamellar structure of PAP248-286; transmission electron microscope was used to observe the effect of lactic acid on the morphology of amyloid fibers; virus infection was used to detect the different time points of incubation with lactic acid. Seminal amyloid fiber promoted the ability of virus infection, after incubating 2 mg/m L PAP248-286 polypeptide with different concentrations of healthy female vaginal secretions, The inhibitory effect of vaginal discharge on amyloid formation in semen was evaluated by sulfur T and Congo red staining. Results Lactic acid could inhibit the formation of amyloid fiber in semen in a concentration-dependent manner. Within 48 h, 0.5 mg/m L lactic acid could inhibit the formation of 2 mg/m L amyloid fiber. Within 48 h, 1 mg/m L lactic acid could inhibit the formation of 尾 -lamellar structure in semen amyloid fiber (2 mg/m L), and 1 mg/m L lactic acid could completely inhibit the polymerization of 2 mg/m L PAP248-286 to form amyloid fiber. The polypeptide PAP248-286, which was incubated with lactic acid, lost the ability to promote virus infection, and the vaginal secretion inhibited the formation of SEVI in a concentration-dependent manner within 48 hours. PAP248-286, 2 mg/m L, had almost no SEVI production in the presence of 67% vaginal secretions. Conclusion Lactic acid can inhibit the formation of amyloid fiber from semen.
【作者单位】：南方医科大学药学院;南方医科大学南方医院妇产科生殖医学中心;
【基金】：国家自然科学基金(31370781) 广州市珠江科技新星(201610010132)~~
【分类号】：R512.91

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