P53和高迁移率族蛋白-1在不同程度慢性乙型肝炎后肝纤维化组织中的含量及其意义

发布时间：2018-06-13 02:19

本文选题：高迁移率族蛋白- + P　；参考：《中南大学学报(医学版)》2015年11期

【摘要】：目的:探讨P53和高迁移率族蛋白-1(high mobility group protein 1,HMGB1)表达在慢性乙型肝炎肝纤维化中的作用。方法:将103例经病理活检证实为肝纤维化的慢性乙型肝炎患者按照非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)量表进行肝纤维化评分,分为3组:无纤维化组(n=18)、低纤维化组(n=49)和高纤维化组(n=36)。检测患者血清HMGB1水平,根据HMGB1水平和肝纤维化的程度绘制受试者工作特征(receiver operating characteristic,ROC)曲线。采用CCl4法诱导肝纤维化大鼠模型后于第6周和第12周处死部分大鼠,Masson染色法观察各组大鼠肝纤维化程度,Western印迹测定大鼠肝组织中HMGB1和P53蛋白水平,酶联免疫吸附法检测组蛋白去乙酰化酶活性以及外周血炎症因子TNF-α,IL-1β,IL-6含量。结果:低纤维化组及高纤维化组患者血清HMGB1水平均明显高于无纤维化组(P0.01);ROC曲线示血清HMGB1诊断肝纤维化程度的cut off值为74 pg/m L,特异性为65%,敏感性为87%。与正常组比较,低、高度肝纤维化组大鼠肝组织蛋白中,核内HMGB1减少,胞质HMGB1增多,而P53蛋白表达量明显增加(P0.01),组蛋白去乙酰化酶活性降低,外周血炎症因子TNF-α,IL-1β,IL-6水平升高。结论:慢性乙型肝炎后肝纤维化组织中P53和HMGB1表达均明显增加;HMGB1水平与肝纤维化水平呈正相关,可作为辅助诊断肝纤维化的指标。
[Abstract]:Aim: to investigate the role of p53 and high mobility group protein 1 HMGB1 in liver fibrosis of chronic hepatitis B (CHB). Methods: 103 patients with chronic hepatitis B confirmed by pathological biopsy were divided into 3 groups according to the non-alcoholic fatty liver disease (NAF LDD) scale: no fibrosis group (n = 18), low fibrosis group (n = 49) and high fibrosis group (n = 36). The serum HMGB1 level was measured and the receiver operating characteristic curve was drawn according to the HMGB1 level and the degree of hepatic fibrosis. The liver fibrosis model of rats was induced by CCL 4 method. After 6 weeks and 12 weeks, some rats were killed to observe the degree of hepatic fibrosis. Western blot was used to detect the levels of HMGB1 and p53 protein in rat liver tissue. Enzyme linked immunosorbent assay (Elisa) was used to detect the activity of histone deacetylase and the content of TNF- 伪 and IL-1 尾 IL-6 in peripheral blood. Results: the serum levels of HMGB1 in low fibrosis group and high fibrosis group were significantly higher than those in non-fibrosis group. The cut off value of serum HMGB1 in diagnosing hepatic fibrosis was 74 pg/m / L, the specificity was 65 and the sensitivity was 87%. Compared with normal group, HMGB1 in nucleus and HMGB1 in cytoplasm were decreased in high hepatic fibrosis group, while p53 protein expression was significantly increased, histone deacetylase activity was decreased, and IL-6 level of peripheral blood inflammatory factor TNF- 伪 was increased in rats with high hepatic fibrosis (HMGB1) and cytoplasmic HMGB1 (HMGB1). Conclusion: the expression of p53 and HMGB1 in liver fibrosis tissues after chronic hepatitis B is significantly increased and the level of HMGB1 is positively correlated with the level of hepatic fibrosis, which can be used as a marker for the diagnosis of hepatic fibrosis.
【作者单位】：广西省柳州市人民医院感染病科;
【分类号】：R512.62;R575.2

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