RFFIT、FAVN、ELISA及狂犬病中和抗体检测试剂盒对狂犬病抗体检测的比较研究

发布时间：2018-06-13 22:07

本文选题：RVNA + Kit　；参考：《病毒学报》2017年02期

【摘要】：为了比较RFFIT、FAVN、ELISA及狂犬病中和抗体检测试剂盒(RVNA Kit)等四种方法对狂犬病抗体的检测效果,对65份狂犬病疫苗免疫前、免疫后临床血清样本,分别使用RFFIT、FAVN、ELISA及RVNA Kit方法对狂犬病病毒特异性抗体进行测定,并对检测结果的阳性符合率、相关性及重复性进行分析。检测结果显示,RFFIT、FAVN及RVNA kit的阳性符合率为100%,三种方法与ELISA试剂盒的阳性符合率为95.38%,对0.5IU/mL~10IU/mL的血清样本进行阳性符合率分析显示,四种方法的阳性符合率为100%;相关性研究结果表明,RFFIT、FAVN及RVNA Kit之间任意两种方法检测结果的相关系数均超过0.97,但ELISA与其他三种方法检测结果的相关系数均低于0.86;同一份样品的重复性检验结果显示,RVNA Kit和ELISA比RFFIT和FAVN具有更好的重复性,而RVNA Kit的重复性最好,其变异系数仅6%。研究结果表明,RFFIT、FAVN及RVNA Kit等三种方法可通用于血清中抗狂犬病病毒中和抗体的检测,而ELISA检测结果与中和抗体效价有一定差距,但在经过优化、校验后ELISA也可以在有效范围内用于狂犬病抗体的检测。
[Abstract]:In order to compare the effect of RFFIT-FAVNU Elisa and RVNA kit in detecting rabies antibody, 65 clinical serum samples were collected before and after immunization with rabies vaccine. RFFIT-FAVNV Elisa and RVNA Kit method were used to detect the specific antibody against rabies virus, and the positive coincidence rate, correlation and repeatability of the results were analyzed. The results showed that the positive coincidence rate of RFFITT FAVN and RVNA kit was 100, the positive coincidence rate of three methods with Elisa kit was 95.38. The positive coincidence rate of 0.5 IUU / mL 10 IUU / mL serum samples was analyzed. The positive coincidence rate of the four methods was 100. The results of correlation study showed that the correlation coefficient of any two methods between RFFITFITFAVN and RVNA Kit exceeded 0.97, but the correlation coefficient between Elisa and other three methods was lower than 0.86. The results of reproducibility test showed that RVNA Kit and Elisa had better repeatability than RFFIT and FAVN. The reproducibility of RVNA Kit was the best, and its coefficient of variation was only 6. The results show that RFFITFITFAVN and RVNA Kit can be used for the detection of neutralizing antibodies against rabies virus in serum. After verification Elisa can also be used in the detection of rabies antibody in the effective range.
【作者单位】：中国疾病预防控制中心病毒病预防控制所;北京博雅晟康医学科技有限公司;
【基金】：国家重点研发计划项目(项目号:2016YFD0500400),题目:重要神经嗜性人兽共患病免疫与致病机制研究国家科技重大专项项目(项目号:2014ZX10004002),题目:艾滋病和病毒性肝炎等重大传染病防治国家科技支撑计划(项目号:2014BAI13B04),题目:狂犬病净化技术集成与人狂犬病消除示范研究国家自然科学基金(项目号:31500152),题目:我国狂犬病病毒种群划分系统的建立
【分类号】：R446.6;R512.99

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