中国5个地区献血人群HCV基因型分布及进化分析

发布时间：2018-06-17 03:23

本文选题：丙型肝炎病毒 + HCV基因　；参考：《中国输血杂志》2017年05期

【摘要】：目的了解国内不同地区献血人群中HCV基因型、亚型分布及系统发育情况以及不同地区人群中HCV流行病毒株。方法收集来自重庆、新疆乌鲁木齐、河南洛阳、四川绵阳和广西柳州5地无偿献血人群的抗-HCV阳性血浆标本802(人)份,采用逆转录巢式PCR对其HCV core基因片段扩增并测序;应用MEGA 6.0软件构建HCV分子进化树并对其做基因分型,用Arlequin和DNAsp软件对不同地区不同HCV基因型做群体动力学研究。采用SPSSStatistics17.0软来分析HCV基因亚型和人口统计学特征的相关性。结果 5个地区献血者抗-HCV阳性血浆标本获得HCV core基因片段成功扩增的比例为52.24%(419/892),其中重庆为47.37%(116/247)、乌鲁木齐为54.17%(104/192)、绵阳为64.35%(74/115)、洛阳为38.95%(67/172)、柳州为76.32%(58/76);所有阳性PCR产物经双向测序后均获得核苷酸序列,共检测出8个HCV基因亚型:HCV-1a 0.72%(3/419)、1b 59.90%(251/419),2a 16.95%(71/419),3a 4.53%(19/419)、3b 3.82%(16/419),6a 12.41%(52/419)、6e 0.95%(4/419)、6n 0.72%(3/419),未检测到HCV-4和5型。5地献血人群中比例较高的2种HCV基因亚型:重庆为1b 46.55%(54/116)、6a18.97%(22/52),乌鲁木齐为1b 67.62%(71/104)、2a 21.90%(22/104)、洛阳为1b 64.18%(43/67)、2a 29.85%(20/67),绵阳为1b 80.00%(60/75)、2a 10.67%(8/75),柳州为1b 41.38%(24/58)、6a 36.21%(21/58)。HCV基因亚型和年龄、民族之间有明显的差异(P0.05)。结论本组我国5个地区献血人群HCV基因亚型丰度较高,以1b为主,2a次之,其余呈散在分布或未检出;不同地区人群的优势基因型均以1b比例为最高,其次分别是2a或6a。HCV基因亚型和年龄、民族存在相关性。
[Abstract]:Objective to understand the HCV genotypes, subtype distribution and phylogeny of blood donors in different regions of China and HCV epidemic strains in different populations. Methods the anti -HCV positive plasma specimens from 5 free blood donors from Chongqing, Urumqi, Xinjiang, Henan, Luoyang, Sichuan Mianyang and Guangxi Liuzhou were collected and the reverse transcriptase was used. The HCV core gene fragment was amplified and sequenced by nested PCR, and MEGA 6 software was used to construct the HCV molecular evolution tree and make a genotyping. The group dynamics of different HCV genotypes in different regions was studied with Arlequin and DNAsp software. The correlation between the HCV gene subtypes and the demographic characteristics was analyzed by SPSSStatistics17.0 soft. Results 5 The successful amplification of HCV core gene fragment was 52.24% (419/892), among which Chongqing was 47.37% (116/247), Urumqi was 54.17% (104/192), Mianyang was 64.35% (74/115), Luoyang was 38.95% (67/172), and Liuzhou was 76.32% (58/76); all positive PCR products were sequenced by bidirectional sequencing. A total of 8 subtypes of HCV genes were detected: HCV-1a 0.72% (3/419), 1b 59.90% (251/419), 2a 16.95% (71/419), 3a 4.53% (19/419), 3b 3.82% (16/419), 6a 12.41% (0.95%), 0.72% (0.72%). 2) Urumqi is 1b 67.62% (71/104), 2a 21.90% (22/104), Luoyang is 1b 64.18% (43/67), 2a 29.85% (20/67), Mianyang is 1b 80% (60/75), 2a 10.67% (8/75), Liuzhou is 41.38%, and there are significant differences among ethnic groups in 5 regions of China. The abundance of type was higher, 1b was dominant, 2a was the second, and the rest were distributed or undetected. The dominant genotypes in different regions were the highest in 1b, followed by 2A or 6a.HCV subtypes and age, and the relationship between ethnic groups.
【作者单位】：中国医学科学院北京协和医学院输血研究所;四川血液安全和血液代用品国际科技合作基地;
【基金】：国家自然科学基金面上项目(81572089)
【分类号】：R512.63

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