I型登革病毒感染后不同病程病毒核酸及其IgM抗体的变化规律

发布时间：2018-06-18 04:04

本文选题：I型登革病毒 + IgM　；参考：《第三军医大学学报》2017年04期

【摘要】：目的探讨I型登革病毒感染后病毒核酸及其IgM抗体在患者体内的变化规律,为指导临床快速、准确地诊断登革热提供理论支持。方法回顾性分析2014年7-11月收集的I型登革病毒感染者临床资料和血清标本,应用实时荧光定量RT-PCR和ELISA法分别检测病毒核酸和IgM抗体,分析不同病程两者的变化规律。结果共检出222例阳性患者,其中病毒核酸阳性137例,IgM抗体阳性146例,两者同时阳性61例。病程急性期(发病1~5 d)病毒核酸阳性率为75%~100%,IgM抗体阳性率在发病第1~2天较低,但第3~5天快速升高至50%~70%;进入恢复期(发病6~10 d)病毒核酸阳性率即快速降低,8 d后仅20%左右,但IgM抗体阳性率显著升高达95%~100%。急性期和恢复期病毒核酸与IgM抗体检测结果构成比差异有统计学意义(χ~2=63.41,P0.05)。结论 I型登革病毒感染者急性期病毒核酸阳性率在75%以上。恢复期IgM抗体阳性率在95%以上。根据病程合理选择检测方法,可提高登革热的诊断效率。
[Abstract]:Objective to investigate the changes of nucleic acid and IgM antibody in patients with dengue virus type I infection, and to provide theoretical support for the rapid and accurate diagnosis of dengue fever. Methods the clinical data and serum samples collected from July to November 2014 were analyzed retrospectively. Real-time quantitative RT-PCR and Elisa were used to detect viral nucleic acid and IgM antibody respectively. Results A total of 222 positive patients were detected, of which 137 were positive for virus nucleic acid and 146 were positive for IgM antibody, 61 of which were positive for both. The positive rate of viral nucleic acid in the acute stage of the disease was 75%. The positive rate of IgM antibody was lower on the 1st and 2nd day of the disease, but rapidly increased to 50% on the 3rd day, and the positive rate of viral nucleic acid in the convalescence stage (6days after onset) decreased rapidly by only about 20% after 8 days. But the positive rate of IgM antibody increased significantly to 95%. There was significant difference in the composition ratio of viral nucleic acid and IgM antibody between acute stage and convalescence stage (蠂 2 + 63.41% P 0.05). Conclusion the positive rate of viral nucleic acid in patients with type I dengue virus in acute stage is more than 75%. The positive rate of IgM antibody in convalescence was over 95%. The diagnosis efficiency of dengue fever can be improved by selecting the detection method reasonably according to the course of disease.
【作者单位】：广州军区疾病预防控制中心;
【基金】：广东省科技计划项目(2014A020219007,2016A020219006,2014A020212252,2015A020218003) 军队科技计划项目(CGZ14J006) 广东省医学科学技术研究基金(A2015571)~~
【分类号】：R512.8

【参考文献】