HBV感染患者病毒载量对外周血T淋巴细胞、细胞因子及肝功能的影响

发布时间：2018-06-18 06:16

本文选题：乙型肝炎病毒 + T淋巴细胞　；参考：《中华医院感染学杂志》2017年01期

【摘要】：目的探讨乙型肝炎病毒(HBV)感染患者病毒载量对外周血T淋巴细胞、细胞因子及肝功能的影响。方法 2015年5月-2016年5月分别采用酶偶联法、流式细胞术和酶联免疫吸附法检测460例HBV感染患者,其中按血清HBV-DNA拷贝数分为阴性组(0~103 copies/ml)96例、低拷贝组(103~105 copies/ml)132例、中拷贝组(105~107 copies/ml)115例和高拷贝组(≥107 copies/ml)117例,对比各组的肝功酶学指标[谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷氨酸转移酶(GGT)]、T淋巴细胞表面标志物(CD4+、CD8+)和细胞因子[白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、促血管生成素-2(Ang-2)和肿瘤坏死因子-α(TNF-α)]水平。结果高拷贝组血清ALT、AST、GGT、IL-2、IL-6和TNF-α水平最高,分别为(231.6±69.2)U/L、(196.4±52.1)U/L、(224.7±62.8)U/L、(19.3±3.2)ng/L、(28.7±5.2)ng/L和(30.1±7.1)ng/L,并且随着HBV-DNA拷贝数降低,水平不断下降;不同HBV-DNA拷贝组CD4+T淋巴细胞、CD8+T淋巴细胞比例比较,差异无统计学意义;经Pearson检验,血清ALT、AST和GGT水平与血清IL-2、IL-6和TNF-α水平呈显著相关性(P0.05)。结论 HBV感染可引起乙型肝炎患者的肝功能损害,但不直接影响T淋巴细胞亚群分布,通过细胞因子IL-2、IL-6和TNF-α水平检测可一定程度上反应肝细胞的损害程度以及机体的免疫状态。
[Abstract]:Objective to investigate the effect of viral load on T lymphocytes, cytokines and liver function of peripheral blood in patients with hepatitis B virus (HBV) infection. Methods in May May 2015, 460 cases of HBV infected patients were detected by enzyme coupling method, flow cytometry and enzyme linked immunosorbent assay (ELISA), which were divided into negative group (0~103 copie) according to the HBV-DNA copy number of serum HBV-DNA. S/ml) 96 cases, 132 cases of low copy group (103~105 copies/ml), 115 cases of medium copy group (105~107 copies/ml) and 117 cases of high copy group (> 107 copies/ml). The liver function enzymology index [ALT), cereal transaminase (AST), glutamate transferase (GGT), T lymphocyte surface markers (CD4+, CD8+) and cytokines [interleukin] were compared. (IL-2), interleukin -6 (IL-6), angiopoietin -2 (Ang-2) and tumor necrosis factor - alpha (TNF- alpha)]. Results the levels of serum ALT, AST, GGT, IL-2, IL-6 and TNF- alpha were highest in the high copy group (231.6 + 69.2), (196.4 + 52.1), (224.7 + 62.8), (19.3 + 3.2), (28.7 + 5.2) and (30.1 + 7.1) There was no significant difference in the proportion of CD4+T lymphocyte and CD8+T lymphocyte in different HBV-DNA copy groups. The levels of serum ALT, AST and GGT were significantly correlated with the level of serum IL-2, IL-6 and TNF- alpha by Pearson test. Conclusion HBV infection could cause liver function damage in patients with hepatitis B, but not straight. The distribution of T lymphocyte subsets is affected by the level of cytokine IL-2, IL-6 and TNF- alpha, and the degree of damage to the liver cells and the immune state of the body can be reacted to some extent.
【作者单位】：郑州铁路职业技术学院医学技术系;郑州铁路职业技术学院护理学院;郑州市第二人民医院检验科;郑州大学第三附属医院·河南省人类精子库;
【基金】：河南省教育厅科科学技术研究重点项目(142202363144)
【分类号】：R512.62

【相似文献】