不同荧光定量PCR技术在乙型肝炎病毒检测中的应用评价

发布时间：2018-06-18 22:55

本文选题：乙型肝炎病毒 + 多重聚合酶链式反应　；参考：《安徽医药》2016年09期

【摘要】：目的评价普通荧光定量PCR技术与罗氏内标法荧光定量PCR技术在乙型肝炎病毒(HBV)检测中的意义。方法采集HBV患者的血清190份,分别用两种方法进行检测,通过相关性分析和Kappa检验评价两种试剂检测结果之间的差异性及一致性。结果 (1)190份标本中,罗氏Cobas试剂与国产试剂检测结果呈线性相关(R2=0.670 7,P0.05);(2)分成HBeAg(+)与HBeAg(-)两组比较,两组中Cobas试剂与国产试剂检测均呈线性相关(R2=0.586 4,P0.05;R2=0.522 6,P0.05);(3)根据不同HBeAg状态和抗病毒要求的DNA载量对标本进行一致性分析,HBeAg(+)组Kappa值为0.752;HBeAg(-)组Kappa值为0.381。结论对于HBeAg(+)患者,可以应用国产试剂检测患者的HBV-DNA水平,而对于HBeAg(-)患者,建议应用Cobas试剂检测其患者的HBV-DNA水平。
[Abstract]:Objective to evaluate the significance of routine fluorescent quantitative PCR (FQ-PCR) and Roche internal standard fluorescence quantitative PCR (FQ-PCR) in the detection of hepatitis B virus (HBV). Methods 190 serum samples from patients with HBV were collected and detected by two methods. The difference and consistency between the two reagents were evaluated by correlation analysis and Kappa test. Results among 190 samples, there was a linear correlation between Cobas reagent and domestic reagent. The results were divided into two groups: HBeAg () and HBeAg- (). There was a linear correlation between Cobas reagent and domestic reagent in both groups. The Kappa value of HBeAg () group was 0.381.The Kappa value of HBeAg () group was 0.381.The consistency analysis was based on different HBeAg status and DNA load required by HBeAg () group (Kappa value was 0.752HBeAg-C), and the Kappa value of HBeAg () group was 0.381.The Kappa value of HBeAg () group was 0.381. Conclusion the HBV-DNA level of HBeAg- (HBeAg-) patients can be detected by domestic reagent, and the HBV-DNA level of HBeAg- (HBeAg-) patients should be detected by Cobas reagent.
【作者单位】：安徽医科大学第一附属医院感染病科;
【基金】：国家自然科学基金项目(81172737)
【分类号】：R512.62;R440

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