可视化核酸恒温扩增技术在日本血吸虫病筛查和诊断中的应用

发布时间：2018-06-22 12:42

本文选题：日本血吸虫 + 核酸提取　；参考：《动物医学进展》2015年12期

【摘要】：日本血吸虫病的筛查和诊断在血吸虫病的防治中处于重要的地位,而核酸诊断是其潜在的优良检测方法。拟建立一套完整的"可视化日本血吸虫核酸恒温扩增检测试剂",用于基层血防单位对日本血吸虫病的筛查和诊断等工作。用腹壁拔毛贴片法给新西兰大白兔感染日本血吸虫,用多种核酸提取方法抽提其血清或血浆中的基因组DNA,并用"可视化日本血吸虫核酸恒温扩增检测试剂"对其进行检测。结果表明,该研究所设计的检测方法可以方便地检测到100条日本血吸虫尾蚴感染的新西兰大白兔,说明可视化核酸恒温扩增技术在日本血吸虫病的筛查和诊断中具有潜在的应用可能性。
[Abstract]:The screening and diagnosis of schistosomiasis japonicum plays an important role in the prevention and control of schistosomiasis, and nucleic acid diagnosis is a potential good detection method. A complete set of "visualized nucleic acid constant temperature amplification reagent" for screening and diagnosis of schistosomiasis japonicum was established. New Zealand white rabbits were infected with Schistosoma japonicum by abdominal wall plucking patch method. The genomic DNA in serum or plasma was extracted by a variety of nucleic acid extraction methods, and the genomic DNA was detected by "visualized Schistosoma japonicum nucleic acid constant temperature amplification reagent". The results showed that 100 New Zealand white rabbits infected with Schistosoma japonicum cercariae could be easily detected by the method designed by the Institute. The results indicate that the visualization nucleic acid isothermal amplification technique has potential application in the screening and diagnosis of schistosomiasis japonicum.
【作者单位】：岳阳职业技术学院医学基础部;湖南康润药业有限公司;军事医学科学院基础医学研究所基因组工程研究室;湖南省血吸虫病防治所免疫诊断研究室;
【基金】：国家重大科技专项(2012ZX10004909) 湖南省科学技术厅科研条件创新专项(2012TT2014)
【分类号】：R532.21

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