新城疫病毒和传染性支气管炎病毒多重DNA微列阵鉴定方法的构建

发布时间：2018-06-23 21:19

  本文选题：新城疫病毒 + 传染性支气管炎病毒　； 参考：《中国病原生物学杂志》2017年08期

【摘要】：目的建立可同时检测新城疫病毒(Newcastle disease virus,NDV)和传染性支气管炎病毒(Infectious bronchitis virus,IBV)的多重DNA微列阵鉴定方法。方法针对NDV和IBV设计PCR引物和微阵列探针,制备检测用微阵列。将样品与微列阵进行杂交,分析微阵列的特异性和灵敏度。结果建立的微阵列检测NDV和IBV为阳性,且能区分NDV和IBV,而流感病毒传染性法氏囊病毒等阴性对照无杂交信号。通过GenePix 4.1软件分析杂交信号的平均SNR532,NDV和IBV的检出限为1×10~3copies/μl。结论建立的多重DNA微列阵鉴定方法具有高特异性和高敏感性,可用于NDV和IBV感染检测。
[Abstract]:Objective to establish a multiplex bronchitis microarray method for simultaneous detection of Newcastle disease virus (NDV) and infectious bronchitis virus (IBV). Methods PCR primers and microarray probes were designed for NDV and IBV to prepare microarrays for detection. The samples were hybridized with microarray to analyze the specificity and sensitivity of the microarray. Results the established microarray was positive for NDV and IBV, and could distinguish NDV from IBV, while the negative control group of Infectious bursal virus (Infectious bursal virus) of influenza virus had no hybridization signal. Using GenePix 4.1 software, the detection limit of average SNR532 NDV and IBV was 1 脳 10~3copies/ 渭 L. Conclusion the multiplex DNA microarray method has high specificity and sensitivity and can be used to detect NDV and IBV infection.
【作者单位】： 长春中药大学附属医院检验科;吉林大学临床医学院;
【基金】：吴阶平医学基金会临床科研专项资助基金课题(No.320.6750.16068)
【分类号】：R440;R511
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本文编号：2058471