负性协同刺激分子PD-1和Tim-3及细胞因子在布鲁氏菌病中的变化

发布时间：2018-06-27 13:25

本文选题：PD- + Tim-　；参考：《免疫学杂志》2017年11期

【摘要】：目的检测布鲁氏菌病患者程序性死亡蛋白1(PD-1)、T细胞免疫球蛋白黏蛋白分子3(Tim-3)及细胞因子IL-17A和IL-6的变化。方法 72例布鲁氏菌患者组与50例正常对照组采用流式细胞术检测外周血单个核细胞中PD-1和Tim-3的表达,实时荧光定量PCR检测外周血单个核细胞中PD-1 mRNA和Tim-3 mRNA的表达,流式液相多重蛋白定量技术(CBA)检测血清中IL-17A和IL-6的水平,Pearson法分析布鲁氏菌病患者外周血中PD-1~+Tim-3~+CD4~+T的表达与IL-17A和IL-6的相关性,分析布鲁氏菌病患者外周血中PD-1~+Tim-3~+CD4~+T的表达及临床特点。结果与正常对照组相比,布鲁氏菌病患者组PD-1~+Tim-3~+CD4~+T细胞、PD-1 mRNA和Tim-3 mRNA的表达、IL-17A和IL-6的水平均明显增加(P0.01),布鲁氏菌病患者外周血中PD-1~+Tim-3~+CD4~+T的表达与IL-17A(r=0.71,P0.01)和IL-6(r=0.83,P0.01)的水平均呈明显的正相关,并且布鲁氏菌病患者出现发热、骨关节痛、多器官受累时外周血中PD-1~+Tim-3~+CD4~+T的表达明显增高(P0.01)。结论 PD-1与Tim-3在布鲁氏菌病患者中表达增加,可能共同参与了布鲁氏菌对免疫应答的负性调控,不利于机体对布鲁氏菌的有效清除。
[Abstract]:Objective to detect the changes of programmed death protein 1 (PD-1) T cell immunoglobulin mucin molecule 3 (Tim-3) and cytokines IL-17A and IL-6 in patients with brucellosis. Methods the expression of PD-1 and Tim-3 in peripheral blood mononuclear cells was detected by flow cytometry in 72 patients with brucella and 50 normal controls. The expression of PD-1 mRNA and Tim-3 mRNA in peripheral blood mononuclear cells was detected by real-time fluorescence quantitative PCR. The levels of IL-17A and IL-6 in serum were detected by flow cytometry (CBA). Pearson method was used to analyze the correlation between the expression of PD-1 ~ Tim-3 ~ CD4~ T and the levels of IL-17A and IL-6 in peripheral blood of patients with brucellosis. The expression and clinical characteristics of PD-1 ~ Tim-3 ~ CD4 ~ T in peripheral blood of patients with brucellosis were analyzed. Results compared with the normal control group, The expression of PD-1 mRNA and Tim-3 mRNA in PD-1 ~ Tim-3 ~ CD4T cells and the levels of IL-17A and IL-6 in peripheral blood of patients with brucellosis were significantly increased (P0.01). The expression of PD-1 ~ Tim-3CD4T in peripheral blood of patients with brucellosis was positively correlated with the levels of IL-17A (r0.71P0.01) and IL-6 (rm0.83P0.01). The expression of PD-1 ~ Tim-3 ~ CD4T in peripheral blood of patients with brucellosis was significantly higher than that of patients with brucellosis (P0.01). Conclusion the increased expression of PD-1 and Tim-3 in patients with brucellosis may participate in the negative regulation of the immune response of brucellosis and is not conducive to the effective clearance of brucellosis.
【作者单位】：新疆医科大学基础医学院免疫学教研室;新疆医科大学第一附属医院感染科;新疆医科大学第一附属医院检验科;
【基金】：国家自然科学基金项目(81560322) 新疆维吾尔自治区自然科学基金(2015211C100) 新疆医科大学科学创新基金(XYDCX201507)
【分类号】：R516.7

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