徐州地区结核分枝杆菌基因分型及其与利福平耐药基因突变的关系

发布时间：2018-06-28 03:24

本文选题：结核分枝杆菌 + 基因分型　；参考：《广东医学》2017年02期

【摘要】：目的探究徐州地区结核分枝杆菌的基因型分布特征及其与利福平耐药基因突变形成的关系。方法选取徐州市传染病医院结核病实验室基因型毒株500株,其中含有经罗氏比例法鉴定的耐利福平临床分离株300株和利福平敏感200株,采用间隔区寡核苷酸分型(spoligotyping)分析其基因型结构及其与利福平抗性基因的突变关系。结果徐州地区的结核分枝杆菌的基因分型主要为北京家族基因群(61.2%)和非北京家族基因群(38.8%),利福平耐药和敏感组北京家族基因群的构成比差异无统计学意义(醊2=0.09,P0.05);北京家族531位点、516位点以及531和526位点突变率分别为55.49%、10.99%和7.69%与非北京家族33.90%、21.19%和15.25%相比,差异有统计学意义(P0.05)。结论徐州地区结核分枝杆菌基因型为北京家族基因群和非北京家族基因群,非北京家族基因群具有基因多态性,两者与利福平耐药率无明显相关,两者在单531、单516以及531和526利福平耐药位点突变率存在差异。
[Abstract]:Objective to investigate the genotypic distribution of Mycobacterium tuberculosis in Xuzhou and its relationship with rifampicin resistance gene mutation. Methods 500 strains of genotypic strains of tuberculosis were selected from Xuzhou Infectious Disease Hospital, including 300 strains of rifampin resistant clinical isolates and 200 strains of rifampicin sensitive strains identified by Roche scale method. The genotype structure and its relationship with rifampicin resistance gene were analyzed by spacer oligonucleotide typing (spoligotyping). Results the main genotypes of Mycobacterium tuberculosis in Xuzhou area were Beijing family gene group (61.2%) and non-Beijing family gene group (38.8%). There was no significant difference between rifampicin resistance group and sensitive group (P 0.05). The mutation rates of 531, 531 and 526 loci in Beijing family were 55.49% and 7.69%, respectively, compared with 33.90% and 15.25% in non-Beijing families. The difference was statistically significant (P0.05). Conclusion the genotypes of Mycobacterium tuberculosis in Xuzhou area are Beijing family gene group and non-Beijing family gene group. The gene polymorphism of non-Beijing family gene group has no significant correlation with rifampin resistance rate. The mutation rates of rifampin resistance loci in single 531, single 516 and 531 and 526 rifampicin were different between the two groups.
【作者单位】：徐州市传染病医院结核二科;
【基金】：徐州市科技计划项目(编号:KC14SH055)
【分类号】：R52

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