CD40、CD40L、IFN-γ、IL-2水平与尖锐湿疣相关性研究

发布时间：2018-06-28 23:03

本文选题：尖锐湿疣 + CD　；参考：《中国艾滋病性病》2017年11期

【摘要】：目的研究尖锐湿疣(CA)高危人群外周血白细胞分化抗原40(CD40)、白细胞分化抗原40配体(CD40L)、干扰素γ(IFN-γ)、白介素2(IL-2)水平,并探讨其与发病的相关性。方法 50例尖锐湿疣门诊患者的性伴及配偶,按是否发病分为患病组(13例)及未患病组(37例),加健康对照组30例,分别抽取外周血分离外周血单核细胞(PBMC),利用流式细胞术检测CD40、CD40L阳性率;利用酶联免疫吸附试验(ELISA)检测血清中IFN-γ、IL-2水平。结果 50例CA患者性伴及配偶中13例发病,5例人乳头瘤病毒(HPV)亚临床感染,共18例HPV脱氧核糖核酸(DNA)阳性,阳性率36%。患病组外周血中CD40、CD40L阳性率及IFN-γ、IL-2水平分别是(7.83±1.25)%、(1.32±0.25)%、(6.13±1.48)pg/m L、(18.24±4.68)pg/m L,明显低于未患病组及健康对照组。结论尖锐湿疣患者性伴及配偶是发病高危人群,CA高危人群发病过程中CD40-CD40L系统起着很重要的防卫作用。
[Abstract]:Objective to study the levels of peripheral leukocyte differentiation antigen 40 (CD40), leukocyte differentiation antigen 40 ligand (CD40L), interferon 纬 (IFN- 纬) and interleukin-2 (IL-2) in high risk population of condyloma acuminatum (CA), and to explore the correlation between CD40, CD40L, IFN- 纬 and IL-2. Methods sexual partners and spouses of 50 patients with condyloma acuminatum were divided into two groups according to the incidence of condyloma acuminatum: 13 cases were in the disease group (13 cases) and 37 cases were in the control group (37 cases), and 30 cases in the healthy control group. Peripheral blood mononuclear cells (PBMC) were isolated from peripheral blood respectively. The positive rate of CD40 and CD40L was detected by flow cytometry, and the level of IL-2 in serum was detected by enzyme-linked immunosorbent assay (Elisa). Results among 50 patients with CA, 13 had subclinical infection of human papillomavirus (HPV), 18 of them were HPV DNA positive, the positive rate was 36. The positive rate of CD40T CD40L and the level of IFN- 纬 IL-2 in peripheral blood were (7.83 卤1.25), (1.32 卤0.25), (6.13 卤1.48) pg/m / L, (18.24 卤4.68) pg/m / L, respectively. Conclusion the sexual partners and spouses of condyloma acuminatum patients are the high risk population of CA and the CD40-CD40L system plays an important role in defense.
【作者单位】：镇江市第一人民医院新区分院;江苏大学附属医院;
【基金】：国家自然科学基金(81573053) 江苏省“333工程”培养资金资助项目(BRA2015196) 镇江市免疫性皮肤病重点实验室项目(ss2015024) 镇江市卫生科技重点专项项目(SHW2016002) 江苏省妇幼健康科研项目(F201717)~~
【分类号】：R752.53

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