荧光定量PCR检测戊肝病毒的应用价值探究

发布时间：2018-07-02 23:21

本文选题：荧光定量聚合酶链反应 + 酶联免疫吸附测定实验　；参考：《现代预防医学》2015年22期

【摘要】：目的探讨荧光定量PCR检测HEV RNA的应用价值。方法从110 535份血清标本中,随机抽取标本200份,分别进行ELISA检验和荧光定量PCR分析。结果 69例ELISA阳性的标本经荧光定量PCR检测均为阳性,31例ELISA可疑的标本经荧光PCR检测也均为阳性,100例ELISA阴性的标本中有2例经荧光定量PCR检测结果为阳性。经Kappa检验,Kappa值为0.72,可认为2种方法检测结果具有中度一致性。采用Stuart-Maxwell检验,结果显示PCR与ELISA检出3种情况的边际概率不相等(χ2=33,P=0.00,P0.05),提示2种方法检测结果存在差异,即荧光PCR的阳性检出率更高。结论荧光定量PCR检测HEV RNA能客观地反映HEV感染、复制及病程变化,修正或补充对ELISA测定结果的判定和解释,有利于HEV感染的早期诊断。
[Abstract]:Objective to investigate the application value of fluorescence quantitative PCR for the detection of HEV RNA. Methods 200 samples were randomly selected from 110535 serum samples, and ELISA test and fluorescence quantitative PCR analysis were performed respectively. Results 69 cases of ELISA positive specimens were detected by fluorescence quantitative PCR, 31 cases of ELISA suspicious specimens were also positive by fluorescence PCR detection, 100 cases of E. Of the LISA negative specimens, 2 cases were tested positive by fluorescence quantitative PCR. The Kappa value was 0.72 by Kappa test, and the results of the 2 methods had moderate consistency. The Stuart-Maxwell test showed that the marginal probability of the 3 cases detected by PCR was not equal (x 2 =33, P=0.00, P0.05), suggesting the existence of the 2 methods. The difference, that is, the positive detection rate of the fluorescent PCR is higher. Conclusion the fluorescence quantitative PCR detection of HEV RNA can objectively reflect the HEV infection, the replication and the change of the course of the disease, and modify or supplement the determination and interpretation of the results of ELISA determination, which is beneficial to the early diagnosis of HEV infection.
【作者单位】：成都市锦江区疾病预防控制中心;美国科罗拉多州立大学;
【分类号】：R440;R512.6

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