抑制Kupffer细胞功能减轻日本血吸虫病肝脏肉芽肿炎症反应
发布时间：2018-07-14 11:41
【摘要】：目的通过制备氯磷酸二钠脂质体(LE-Cl2MDP),选择性剔除日本血吸虫感染小鼠模型中Kupffer细胞,研究抑制Kupffer细胞功能对日本血吸虫肝脏肉芽肿形成的影响。方法采用脂质体包裹CL2MDP;6~8周龄雌性BALB/c小鼠分为3组:PBS组,日本血吸虫感染组,日本血吸虫感染联合LE-Cl2BMP组;对于联合组,小鼠尾静脉注射LE-Cl2MDP(0.1mL/10g),每周2次,连续6周;感染后第12周,剥杀小鼠,留取血清及肝脏,用于细胞因子和免疫组织化学检测等,数据统计分析。结果经过LE-Cl2MDP处理后,日本血吸虫感染肉芽肿周围Kupffer细胞细胞明显减少;无论是感染第8周,还是第12周肝组织中虫卵肉芽肿周围炎症细胞浸润明显减少,纤维化程度减轻;同时降低感染第12周小鼠血清IL-10水平,LE-Cl2MDP组为(42.6±10.4)pg/mL,感染组为(67.4±12.9)pg/mL(P0.05)。结论本研究建立LE-Cl2MDP剔除小鼠肝脏Kupffer细胞的日本血吸虫感染模型,抑制了肝组织中虫卵肉芽肿周围炎症反应。
[Abstract]:Objective to study the effect of inhibition of Kupffer cell function on the formation of hepatic granuloma of Schistosoma japonicum by preparing LE-Cl2MDP and selectively culling Kupffer cells from Schistosoma japonicum (Schistosoma japonicum) model. Methods female BALB / c mice were divided into three groups by liposome-encapsulated CL2MDP 6week-old female BALB / c mice into three groups: Schistosoma japonicum infection group and LE-Cl2BMP group; for the combined group, mice were injected with LE-Cl2MDP (0.1 mL / 10 g) twice a week, for 6 weeks; 12 weeks after infection, the mice were treated with LE-Cl2MDP (0.1 mL / 10 g) twice a week. The mice were stripped, serum and liver were retained for cytokine and immunohistochemical detection, and statistical analysis of data was made. Results after treatment with LE-Cl2MDP, the Kupffer cells around the granuloma infected by Schistosoma japonicum decreased significantly, and the infiltration of inflammatory cells around the egg granuloma decreased significantly and the degree of fibrosis decreased in the liver tissue at the 8th and 12th week of infection. At the same time, the serum IL-10 level was decreased in LE-Cl2MDP group (42.6 卤10.4) PG / mL, and in the infected group was (67.4 卤12.9) PG / mL (P0.05). Conclusion the model of Schistosoma japonicum infection with Kupffer cells in liver of LE-Cl2MDP knockout mice was established and the inflammatory reaction around egg granuloma in liver tissue was inhibited.
【作者单位】： 皖南医学院弋矶山医院感染性疾病科;
【基金】：国家自然科学基金(81141083) 安徽省高校省级自然科学研究重点项目(KJ2014A271)~~
【分类号】：R532.21
[Abstract]:Objective to study the effect of inhibition of Kupffer cell function on the formation of hepatic granuloma of Schistosoma japonicum by preparing LE-Cl2MDP and selectively culling Kupffer cells from Schistosoma japonicum (Schistosoma japonicum) model. Methods female BALB / c mice were divided into three groups by liposome-encapsulated CL2MDP 6week-old female BALB / c mice into three groups: Schistosoma japonicum infection group and LE-Cl2BMP group; for the combined group, mice were injected with LE-Cl2MDP (0.1 mL / 10 g) twice a week, for 6 weeks; 12 weeks after infection, the mice were treated with LE-Cl2MDP (0.1 mL / 10 g) twice a week. The mice were stripped, serum and liver were retained for cytokine and immunohistochemical detection, and statistical analysis of data was made. Results after treatment with LE-Cl2MDP, the Kupffer cells around the granuloma infected by Schistosoma japonicum decreased significantly, and the infiltration of inflammatory cells around the egg granuloma decreased significantly and the degree of fibrosis decreased in the liver tissue at the 8th and 12th week of infection. At the same time, the serum IL-10 level was decreased in LE-Cl2MDP group (42.6 卤10.4) PG / mL, and in the infected group was (67.4 卤12.9) PG / mL (P0.05). Conclusion the model of Schistosoma japonicum infection with Kupffer cells in liver of LE-Cl2MDP knockout mice was established and the inflammatory reaction around egg granuloma in liver tissue was inhibited.
【作者单位】： 皖南医学院弋矶山医院感染性疾病科;
【基金】：国家自然科学基金(81141083) 安徽省高校省级自然科学研究重点项目(KJ2014A271)~~
【分类号】：R532.21
【参考文献】
相关期刊论文 前4条
1 杨江华;贺蕾;王涛;杨进孙;侯为顺;苏川;;Kupffer细胞对日本血吸虫病肉芽肿期CD4~+CD25~+T细胞的影响[J];中国血吸虫病防治杂志;2010年01期
2 吴俊q，
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