唐山市HIV抗体确证试验的替代策略研究

发布时间：2018-07-15 21:02

【摘要】：目的在几种艾滋病病毒(HIV)抗体筛查试验组合中,找出哪种HIV初筛试验组合做蛋白印迹(WB)试验的必要性。方法以2010-2016年,唐山市HIV初筛试验有反应的1 972份需进一步做WB确证试验的样本为研究对象,包括确证结果为"阳性"、"不确定"、"阴性"样本。两种快检(RT)(+)、两种RT(-)、一种RT(+)或(±),分别与各种酶联免疫吸附试验(ELISA)结果、化学发光结果的各种试验组合与WB结果及不确定带型的比较分析。结果1 972份样本中,两种RT(+)与ELISA、化学发光的试验组合中,WB确证阳性860例,不确定8例,阴性0例;两种RT(-)的试验组合中,WB不确定137例,2例随访后转阳,阴性925例;一种RT(+)或(±)的试验组合中,WB确证阳性7例,阴性20例,不确定15例,其中4例转阳;三种不同RT组合试验的差异有统计学意义(x~2=2 581.367,P=0.001)。在两种RT(+)与ELISA、化学发光的7类试验组合与WB结果的比较无统计学意义(x~2=4.850,P=0.563)。结论两种RT(+)两种ELISA S/C(O值均≥6的试验组合替代WB试验确证HIV感染是完全可行的;两种RT(一)与一种ELISA阴性的试验组合是没必要进行WB确证试验的。这种替代策略可以大量降低HIV抗体确证的费用,也减少WB不确定结果带来的不必要的伤害。
[Abstract]:Objective to find out which HIV screening test combination is necessary for Western blot (WB) in several HIV antibody screening test combinations. Methods A total of 1 972 samples from Tangshan City from 2010 to 2016 were selected for further WB confirmatory test, including "positive", "uncertain" and "negative" samples. Two kinds of RT) (), RT (-), one RT () or (卤) were compared with the results of enzyme linked immunosorbent assay (Elisa), chemiluminescence and WB results and uncertain band patterns. Results among the 1 972 samples, 860 cases were confirmed positive, 8 cases were uncertain and 0 cases were negative in two kinds of RT (-) RT (-) and two RT (-) test combinations, 137 cases were confirmed positive, 925 cases were negative after follow up. In one kind of RT () or (卤) combination, 7 cases were confirmed positive, 20 cases were negative, 15 cases were uncertain, among them, 4 cases changed to positive, and the difference of three different RT combination tests was statistically significant (xan2an2 581.367 P0.001). In the two RT () and ELISAs, there was no significant difference between the seven chemiluminescence test combinations and the WB results. Conclusion it is completely feasible to confirm HIV infection by two RT () two Elisa Sr / C (O 鈮，

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