华东地区献血者人群隐匿性乙型肝炎病毒感染及S区变异分析

发布时间：2018-07-26 10:58

【摘要】：目的了解安徽、福建、江西三地血液中心无偿献血者隐匿性乙型肝炎病毒(OBI)感染情况,探讨HBV基因分型特征及S区氨基酸变异特点。方法对2013年1月-2014年5月安徽省血液中心、2013年6月-2013年11月、2014年3月-2014年12月福建省血液中心、2013年6月-2013年9月江西省血液中心乙肝表面抗原酶联免疫法检测阴性(HBs Ag-)、核酸HBV鉴别试验阳性(NAT+)的102例无偿献血者标本进行抗-HBc检测,同时应用巢式PCR方法对这些标本进行HBV S区扩增并测序,采用MEGA 6软件进行HBV基因分型及S区氨基酸突变分析。结果在安徽省血液中心123 046例无偿献血者中筛查出21例HBs Ag-NAT+无偿献血者标本,OBI感染率为0.017%(21/123 046),其中76.2%(16/21)为抗-HBc阳性,这21例标本中,15例扩增出HBV S区片段,8例B型,7例C型;福建省血液中心HBs Ag-NAT+无偿献血者标本共51例,76.5%(39/51)为抗-HBc阳性,其中16例扩增出HBV S区片段,14例B型,2例C型;江西省血液中心HBs Ag-NAT+献血者标本共30例,80%(24/30)为抗-HBc阳性,其中4例扩增出HBV S区片段,1例B型,3例C型。35例扩增产物的OBI标本中,74.3%(26/35)出现HBV S基因MHR区氨基酸置换突变,主要集中在乙型肝炎病毒"α"决定簇区域。结论安徽地区无偿献血者OBI感染率为0.017%,福建及江西地区也有一定OBI感染;成功扩增HBV S区的OBI标本中,抗-HBc阳性率为安徽73.3%、福建93.8%、江西100%;B型为主要HBV基因型;HBV S基因MHR区变异,尤其是HBs Ag"α"决定簇区置换突变,可能是OBI形成原因之一。
[Abstract]:Objective to investigate the occult hepatitis B virus (OBI) infection in blood donors from three blood centers in Anhui, Fujian and Jiangxi, and to explore the characteristics of HBV genotyping and amino acid variation in S region. Methods from January 2013 to May 2014, from June 2013 to November 2013, from March 2014 to December 2014, from June 2013 to May 2014, from March 2014 to December 2014, from June 2013 to September 2013, hepatitis B surface antigen enzyme linked immunosorbent assay (Elisa) was used to detect hepatitis B surface antigen. HBs Ag-, DNA HBV positive (NAT) samples from 102 volunteer blood donors were tested for anti-HBc. At the same time, nested PCR method was used to amplify and sequence the S region of HBV. HBV genotyping and amino acid mutation analysis of S region were carried out by MEGA 6 software. Results the infection rate of Obi was 0.017% (21 / 123,046) in the 123046 volunteer blood donors from Anhui Blood Center, of which 76.2% (16 / 21) were positive for anti-HBc. In 15 of the 21 samples, 8 cases of B type C were amplified from HBV S region. There were 51 (76.5%) (39 / 51) anti-HBc positive samples from HBs Ag-NAT donors in Fujian blood center, of which 16 cases were amplified from HBV S region in 14 cases with type B and 2 cases with type C, and 30 cases (80%) (24 / 30) of HBs Ag-NAT blood donors from Jiangxi Blood Center were anti-HBc positive, the positive rate of anti-HBc was 80% (24 / 30). The amino acid replacement mutation in MHR region of HBV S gene was found in 74.3% (26 / 35) of 4 cases of OBI samples with HBV S region amplified from 3 cases of type B type C and 35 cases of type C amplification products, mainly concentrated in the "伪" determinant region of hepatitis B virus. Conclusion the infection rate of OBI was 0.017 in free blood donors in Anhui, and there were some OBI infections in Fujian and Jiangxi, and the positive rate of anti-HBc was 73.3% in Anhui province, 93.8% in Fujian province, and 100% in Jiangxi province. The positive rate of anti-HBc was 73.3% in Anhui, 93.8% in Fujian province, and HBV% in Jiangxi province. In particular, the substitution mutation of HBs Ag "伪" determinant region may be one of the reasons for the formation of OBI.
【作者单位】：安徽省血液中心;安徽医科大学第四附属医院;福建省血液中心;江西省血液中心;
【分类号】：R193.3;R512.62

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