Toll样受体9基因多态性与HBV感染自发清除及肝脏炎症活动的相关性研究
发布时间：2018-08-11 15:54
【摘要】：目的：研究探讨Toll样受体9(TLR9)单核苷酸多态性与HBV感染自发清除与肝脏炎症活动的相关性。 方法：收集422名慢性乙型肝炎病毒(hepatitis B virus, HBV)感染者(其中包括有149名乙型肝炎携带者、178名慢性乙型肝炎患者以及95名慢性重型乙型肝炎患者)和174名HBV感染自发清除者,采用TaqMan探针及PCR技术,检测出TLR9rs187084、rs352139、 rs352140、rs5743836位点的SNPs,分析各组间基因型及等位基因分布是否存在差异；并将慢性HBV感染组按疾病进展、炎症程度、HBV-DNA载量进行分组,比较各组间基因型及等位基因分布时候存在差异；并研究这四个位点是否存在连锁不平衡。 结果：TLR9rs187084、rs352139、rs352140三个位点在HBV携带者与HBV感染自发清除者之间,其基因型及等位基因分布存在差异(P0.05)。rs187084位点TT、TC基因型和T等位基因的频率HBV感染自发清除者明显高于慢性HBV感染组(P0.05)。在慢性HBV感染患者中rs187084位点TT、TC基因型和T等位基因的频率慢性乙型肝炎组及慢性重型肝炎组明显高于HBV携带者(P0.05),rs352139位点AA基因型和A等位基因、rs352140位点CC基因型和C等位基因的频率慢性乙型肝炎组明显高于HBV携带者组。未发现rs187084、rs352139、rs352140三个位点SNP分型与肝脏炎症程度及血清HBV-DNA载量高低有关联。研究还发现rs5743836位点在本研究人群中不存在基因多态性。rs187084、rs352139、 rs352140三个位点之间存在连锁不平衡。 结论：1、TLR9基因rs187084、rs35213、rs352140位点基因多态性与HBV感染的免疫清除功能及肝脏炎症活动有关,但未发现该三个位点的基因多态性与肝脏炎症严重程度及HBV-DNA载量高低有关联。2、TLR9基因rs5743836位点可能不存在基因多态性。3、TLR9基因rs187084、rs352139、rs352140位点之间存在连锁不平衡。
[Abstract]:Aim: to investigate the relationship between Toll like receptor 9 (TLR9) single nucleotide polymorphism (SNP) and spontaneous clearance of HBV infection and hepatic inflammation. Methods: 422 patients with chronic hepatitis B virus (hepatitis B virus, HBV) infection (including 149 hepatitis B carriers, 178 patients with chronic hepatitis B and 95 patients with chronic severe hepatitis B) and 174 patients with spontaneous clearance of HBV infection were collected. TaqMan probes and PCR techniques were used to detect the SNPs at the sites of TLR9rs187084, rs352139, rs352140 and rs5743836, and to analyze whether there were differences in the distribution of genotypes and alleles among the groups. The genotypes and alleles of the four loci were compared, and the linkage disequilibrium of the four loci was studied. Results the genotype and allele distribution of TLR9rs187084 rs352139rs352140 in HBV carriers and HBV infected spontaneous clearance patients were significantly higher than that in chronic HBV infected patients (P0.05). Rs187084 locus TTT TC genotype and T allele spontaneous clearance frequency were significantly higher than those in chronic HBV infection group (P0.05). The frequencies of TTTC genotype and T allele of rs187084 locus in patients with chronic HBV infection were significantly higher in chronic hepatitis B group and chronic severe hepatitis group than in HBV carriers (P0.05). The frequency of alleles in chronic hepatitis B patients was significantly higher than that in HBV carriers. No correlation was found among the three SNP types of rs187084 rs352139rs352140 and the degree of liver inflammation and the level of serum HBV-DNA load. It was also found that there was no gene polymorphism in the rs5743836 locus. Rs187084 rs352139, and the linkage disequilibrium existed among the three rs352140 loci. Conclusion the rs187084 rs35213rs352140 polymorphism of the TLR9 gene is associated with the immune clearance function of HBV infection and the inflammatory activity of the liver. However, no association was found between the polymorphism of the three loci and the severity of liver inflammation and the level of HBV-DNA load. There may be no linkage disequilibrium between the rs5743836 locus of the TLR9 gene and the rs187084rs352139rs352140 locus.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R512.62
本文编号：2177458
[Abstract]:Aim: to investigate the relationship between Toll like receptor 9 (TLR9) single nucleotide polymorphism (SNP) and spontaneous clearance of HBV infection and hepatic inflammation. Methods: 422 patients with chronic hepatitis B virus (hepatitis B virus, HBV) infection (including 149 hepatitis B carriers, 178 patients with chronic hepatitis B and 95 patients with chronic severe hepatitis B) and 174 patients with spontaneous clearance of HBV infection were collected. TaqMan probes and PCR techniques were used to detect the SNPs at the sites of TLR9rs187084, rs352139, rs352140 and rs5743836, and to analyze whether there were differences in the distribution of genotypes and alleles among the groups. The genotypes and alleles of the four loci were compared, and the linkage disequilibrium of the four loci was studied. Results the genotype and allele distribution of TLR9rs187084 rs352139rs352140 in HBV carriers and HBV infected spontaneous clearance patients were significantly higher than that in chronic HBV infected patients (P0.05). Rs187084 locus TTT TC genotype and T allele spontaneous clearance frequency were significantly higher than those in chronic HBV infection group (P0.05). The frequencies of TTTC genotype and T allele of rs187084 locus in patients with chronic HBV infection were significantly higher in chronic hepatitis B group and chronic severe hepatitis group than in HBV carriers (P0.05). The frequency of alleles in chronic hepatitis B patients was significantly higher than that in HBV carriers. No correlation was found among the three SNP types of rs187084 rs352139rs352140 and the degree of liver inflammation and the level of serum HBV-DNA load. It was also found that there was no gene polymorphism in the rs5743836 locus. Rs187084 rs352139, and the linkage disequilibrium existed among the three rs352140 loci. Conclusion the rs187084 rs35213rs352140 polymorphism of the TLR9 gene is associated with the immune clearance function of HBV infection and the inflammatory activity of the liver. However, no association was found between the polymorphism of the three loci and the severity of liver inflammation and the level of HBV-DNA load. There may be no linkage disequilibrium between the rs5743836 locus of the TLR9 gene and the rs187084rs352139rs352140 locus.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R512.62
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