新疆阿勒泰山地蜱传脑炎疫源地调查
发布时间：2018-08-14 17:18
【摘要】：目的调查新疆阿勒泰山地蜱传脑炎疫源地特征,分离鉴定蜱传脑炎病毒。方法通过家畜体表捡法采集寄生蜱;利用间接免疫荧光法检测当地健康人群血清中蜱传脑炎病毒IgG抗体;通过将蜱研磨液接种实验小鼠进行病原动物分离;通过接种BHK-21细胞对蜱传脑炎病毒进行分离培养;利用RT-PCR方法对病毒E蛋白基因片段进行扩增和测序,通过序列分析明确病毒系统进化特征。结果新疆阿勒泰山地白哈巴地区分布有2种蜱,森林革蜱为优势种(55.6%),其次为边缘革蜱(44.4%);当地人群蜱传脑炎IgG抗体阳性率5.31%(6/113);通过动物试验和细胞分离培养,从森林革蜱中分离出一株森林脑炎病毒;对病毒E蛋白基因序列的系统进化分析表明,蜱传脑炎病毒阿勒泰分离株属于远东亚型。结论首次从病原学上证实新疆阿勒泰山地存在蜱传脑炎疫源地,媒介为森林革蜱,病毒流行株为远东亚型。
[Abstract]:Objective to investigate the characteristics of tick-borne encephalitis foci in Altay Mountain, Xinjiang, and to isolate and identify tick-borne encephalitis virus. Methods the parasitic ticks were collected by the method of animal surface collection, the IgG antibody of tick-borne encephalitis virus was detected by indirect immunofluorescence method, and the pathogenic animals were isolated by inoculating ticks grinding fluid into experimental mice. The tick-borne encephalitis virus was isolated and cultured by inoculating BHK-21 cells, and the viral E protein gene fragment was amplified and sequenced by RT-PCR method, and the phylogenetic characteristics of the virus were identified by sequence analysis. Results there were two species of ticks in Baekhaba area of Altay Mountain, Xinjiang, the dominant species were forest ticks (55.6%), the next were the marginal ticks (44.4%), the positive rate of IgG antibody against tick-borne encephalitis in local population was 5.31% (6 / 113), and the positive rate was 5.31% (6 / 113). A forest encephalitis virus was isolated from the forest ticks, and the phylogenetic analysis of the E protein gene showed that the Acari encephalitis virus Altay isolate belonged to the far East subtype. Conclusion Acari encephalitis foci exist in Altay mountain area of Xinjiang for the first time, the vector is ticks, and the epidemic strain is far East subtype.
【作者单位】： 新疆军区疾病预防控制中心;新疆军区第十六医院;
【基金】：国家自然科学基金项目(No.U1303104)~~
【分类号】：R512.34
[Abstract]:Objective to investigate the characteristics of tick-borne encephalitis foci in Altay Mountain, Xinjiang, and to isolate and identify tick-borne encephalitis virus. Methods the parasitic ticks were collected by the method of animal surface collection, the IgG antibody of tick-borne encephalitis virus was detected by indirect immunofluorescence method, and the pathogenic animals were isolated by inoculating ticks grinding fluid into experimental mice. The tick-borne encephalitis virus was isolated and cultured by inoculating BHK-21 cells, and the viral E protein gene fragment was amplified and sequenced by RT-PCR method, and the phylogenetic characteristics of the virus were identified by sequence analysis. Results there were two species of ticks in Baekhaba area of Altay Mountain, Xinjiang, the dominant species were forest ticks (55.6%), the next were the marginal ticks (44.4%), the positive rate of IgG antibody against tick-borne encephalitis in local population was 5.31% (6 / 113), and the positive rate was 5.31% (6 / 113). A forest encephalitis virus was isolated from the forest ticks, and the phylogenetic analysis of the E protein gene showed that the Acari encephalitis virus Altay isolate belonged to the far East subtype. Conclusion Acari encephalitis foci exist in Altay mountain area of Xinjiang for the first time, the vector is ticks, and the epidemic strain is far East subtype.
【作者单位】： 新疆军区疾病预防控制中心;新疆军区第十六医院;
【基金】：国家自然科学基金项目(No.U1303104)~~
【分类号】：R512.34
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