IL-10在细粒棘球蚴感染小鼠的免疫调控作用研究

发布时间：2018-08-26 19:13

【摘要】：目的通过检测细粒棘球蚴感染小鼠IL-10表达水平的变化,探讨IL-10的免疫调控作用。方法收集感染细粒棘球蚴病羊肝脏囊泡中的原头蚴,处理后接种实验组小鼠肝脏,建立实验动物模型。于感染后2、8、30、90和270d各取8只小鼠处死,收集血液,分离血清,采用ELISA检测IL-10含量;同时剥取小鼠脾脏,采用RT-PCR检测IL-10mRNA。实验设0.9%NaCl对照组。结果与对照组比较,实验组小鼠在感染后60和90d血清IL-10表达水平显著升高,在感染后180d达到峰值,随后略有下降,但持续高水平表达(t180d=-27.087,t270d=-19.382,均P0.01)。与对照组相比,实验组小鼠脾脏IL-10mRNA水平在感染后90d达到峰值(t=-3.959,P0.01),之后表达量仍然处于较高水平状态(t180d=-2.571,t270d=-3.284,均P0.01)。结论细粒棘球蚴感染后期小鼠IL-10持续高表达,这可能是导致细粒棘球蚴发生免疫逃逸的机制之一。
[Abstract]:Objective to investigate the immune regulation of IL-10 by detecting the expression of IL-10 in mice infected with Echinococcus granulosus. Methods the primordial hydatid in liver vesicles of sheep infected with echinococcus granulosus were collected and inoculated into the liver of the experimental group and the experimental animal model was established. On the 2nd day after infection, 8 mice were killed, blood was collected, serum was isolated, IL-10 content was detected by ELISA, spleen was stripped and IL-10mRNA. was detected by RT-PCR. 0.9%NaCl control group was used. Results compared with the control group, the expression of serum IL-10 in the experimental group increased significantly at 60 and 90 days after infection, reached its peak at 180 days after infection, then decreased slightly, but continued to be high (t180d-27.087t270d-19.382, both P0.01). Compared with the control group, the level of IL-10mRNA in the spleen of the experimental group reached its peak at 90 days after infection (t ~ (-3.959) P _ (0.01), and the expression level was still in a higher level (t _ (180) d ~ (-2.571) ~ (27) d ~ (-1) -3.284 (all P ~ (0.01). Conclusion the overexpression of IL-10 in mice with late infection of Echinococcus granulosus may be one of the mechanisms leading to immune escape of Echinococcus granulosus.
【作者单位】：新疆医科大学第一附属医院新疆重大疾病医学重点实验室-省部共建国家重点实验室培育基地;新疆医科大学基础医学院;
【基金】：国家自然科学基金项目(No.81260253;31160194;30960358;81160200;81060135) 新疆医科大学一附院青年基金项目(No.N2014ZRQN21) 研究生创新基金(No.XJGRI2014097)
【分类号】：R532.32

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