XIAP在尖锐湿疣组织中的表达及其与HPV分型的相关性

发布时间：2018-09-01 08:49

【摘要】：目的探讨X连锁凋亡抑制蛋白(XIAP)在尖锐湿疣组织中的表达及其与人乳头瘤病毒感染的关系。方法免疫组织化学方法分别检测尖锐湿疣和正常包皮组织中XIAP的表达,采用实时聚合酶链式反应体外扩增和反向点杂交相结合的DNA芯片技术对尖锐湿疣组织进行基因分型检测。结果正常包皮组、尖锐湿疣未复发组和复发组中XIAP的阳性表达率依次升高(分别是26.67%、53.49%和79.31%),3者之间两两比较,差异有统计学意义(P0.05);XIAP在高危型HPV组与正常对照组的表达比较,差异有统计学意义(P0.05),高危型HPV组高于对照组;在高危型与低危型组间、低危型与正常对照组间的表达比较,差异无统计学意义(P0.05);XIAP在不同年龄及性别患者间的表达比较,差异无统计学意义(P0.05);而患者不同的病程及复发次数,XIAP阳性表达率比较,差异有统计学意义(P0.05)。结论尖锐湿疣组织中XIAP表达上调可能是其发生和复发的重要原因,XIAP在尖锐湿疣的恶性转化过程中可能起促进作用,对判断患者病情预后具有一定的参考价值。
[Abstract]:Objective to investigate the expression of X-linked inhibitor of apoptosis protein (XIAP) in condyloma acuminatum and its relationship with human papillomavirus (HPV) infection. Methods the expression of XIAP in condyloma acuminatum and normal prepuce tissue was detected by immunohistochemical method. The genotyping of condyloma acuminatum tissue was detected by real-time polymerase chain reaction in vitro amplification and reverse dot hybridization DNA microarray technique. Results the positive expression rate of XIAP in normal prepuce group, non-recurrent condyloma acuminatum group and relapsed group was increased in turn (26.67% and 79.31%, respectively). The difference was statistically significant (P0.05) between the high risk HPV group and the normal control group. The difference was statistically significant (P0.05), the expression of XIAP in high risk HPV group was higher than that in control group, and the expression of XIAP in high risk type and low risk group was not significantly different from that in normal control group (P0.05). The difference was not statistically significant (P0.05), but the positive expression rate of XIAP in patients with different course and recurrence times was significantly different (P0.05). Conclusion the upregulation of XIAP expression in condyloma acuminatum may be an important reason for its occurrence and recurrence. XIAP may play a promoting role in the malignant transformation of condyloma acuminatum. It may be useful in judging the prognosis of condyloma acuminatum.
【作者单位】：山东省交通医院皮肤科;
【分类号】：R752.53

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