转染HBV Creg DNA的小鼠肝细胞表面干性分子表达观察
发布时间：2018-09-06 16:28
【摘要】：目的观察转染pc DNA3.1-HBV Creg DNA质粒的小鼠肝细胞表面干性分子CD44、CD90、CD133的表达变化。方法从含有pc DNA3.1-HBV Creg DNA质粒的Ecoli DH5α菌株中提取pc DNA3.1-HBV Creg DNA质粒。取SPF级正常小鼠的肝细胞,常规培养,待细胞贴壁后,加入50μL pc DNA3.1-HBV Creg DNA混合转染细胞。采用免疫细胞化学染色法检测转染前后小鼠肝细胞表面干性分子CD44、CD90、CD133表达。结果转染前,部分小鼠肝细胞CD90、CD133呈阳性,且阳性染色细胞都是圆形。未见有CD44阳性染色细胞。转染pc DNA3.1-HBV Creg DNA后,小鼠肝细胞表面干性分子CD44、CD90、CD133呈阳性染色。结论转染pc DNA3.1-HBV Creg DNA质粒的小鼠肝细胞表达CD44、CD90、CD133。HBV Creg DNA可能影响小鼠肝细胞表面干性分子CD44的表达。
[Abstract]:Objective to observe the expression of dry molecule CD44,CD90,CD133 on the surface of mouse hepatocytes transfected with pc DNA3.1-HBV Creg DNA plasmid. Methods pc DNA3.1-HBV Creg DNA plasmid was extracted from Ecoli DH5 伪 strain containing pc DNA3.1-HBV Creg DNA plasmid. The hepatocytes of SPF grade normal mice were cultured routinely. After the cells were adhered, 50 渭 L pc DNA3.1-HBV Creg DNA was added into the transfected cells. The expression of dry molecule CD44,CD90,CD133 on hepatocytes of mice before and after transfection was detected by immunocytochemical staining. Results before transfection, some mouse hepatocytes were positive for CD90,CD133, and the positive staining cells were round. No CD44 positive staining cells were found. After transfection of pc DNA3.1-HBV Creg DNA, CD44,CD90,CD133 positive staining was observed on the surface of mouse hepatocytes. Conclusion the expression of CD44,CD90,CD133.HBV Creg DNA in murine hepatocytes transfected with pc DNA3.1-HBV Creg DNA plasmid may affect the expression of dry molecule CD44 on the surface of mouse hepatocytes.
【作者单位】： 黔西南民族职业技术学院医药系;
【基金】：贵州省科技厅科学技术基金资助项目(2013-2268)
【分类号】：R735.7;R512.62
，
本文编号：2226907
[Abstract]:Objective to observe the expression of dry molecule CD44,CD90,CD133 on the surface of mouse hepatocytes transfected with pc DNA3.1-HBV Creg DNA plasmid. Methods pc DNA3.1-HBV Creg DNA plasmid was extracted from Ecoli DH5 伪 strain containing pc DNA3.1-HBV Creg DNA plasmid. The hepatocytes of SPF grade normal mice were cultured routinely. After the cells were adhered, 50 渭 L pc DNA3.1-HBV Creg DNA was added into the transfected cells. The expression of dry molecule CD44,CD90,CD133 on hepatocytes of mice before and after transfection was detected by immunocytochemical staining. Results before transfection, some mouse hepatocytes were positive for CD90,CD133, and the positive staining cells were round. No CD44 positive staining cells were found. After transfection of pc DNA3.1-HBV Creg DNA, CD44,CD90,CD133 positive staining was observed on the surface of mouse hepatocytes. Conclusion the expression of CD44,CD90,CD133.HBV Creg DNA in murine hepatocytes transfected with pc DNA3.1-HBV Creg DNA plasmid may affect the expression of dry molecule CD44 on the surface of mouse hepatocytes.
【作者单位】： 黔西南民族职业技术学院医药系;
【基金】：贵州省科技厅科学技术基金资助项目(2013-2268)
【分类号】：R735.7;R512.62
，
本文编号：2226907
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