珠海市恙虫病东方体基因型研究

发布时间：2018-09-16 22:10

【摘要】：目的对广东省珠海市恙虫病东方体进行基因分型。方法予高热期恙虫病患者静脉采血后,立刻床旁小白鼠腹腔接种,待发病后解剖,取其腹壁刮液涂片、吉姆萨染色镜检;以巢式PCR进行恙虫病东方体DNA检测和序列分析,将结果进行同源性分析。结果床旁小白鼠腹腔接种,腹壁刮液涂片镜检可见紫红色圆形、椭圆形、短杆状恙虫病东方体菌体。10例恙虫病患者进行特异性的巢式PCR扩增,其中6例扩增出目的基因片段,阳性率为60%。将扩得珠海恙虫病东方体基因片段的DNA序列与基因库序列进行比对,发现其与韩国株Yonchon株同源性最高,大于95%,系统进化树显示其与Karp株在同一分支。结论珠海市为恙虫病的自然疫源地,该地区恙虫病东方体的基因型与Yonchon株最相近。
[Abstract]:Objective to study the genotyping of Origamushi tsutsugamushi in Zhuhai, Guangdong Province. Methods after intravenous blood collection from patients with tsutsugamushi disease in high fever stage, the mice were inoculated intraperitoneally immediately beside the bed. After the onset of the disease, the mice were dissected, the scrape smears of the abdominal wall were taken and examined by Gimsa staining, and the DNA and sequence analysis of Orient body of tsutsugamushi disease were performed by nested PCR. The results were analyzed by homology analysis. Results the rodents were inoculated intraperitoneally by the bed, and the smear smears of the abdominal wall showed fuchsia rotund, oval, short rod-like oriental bacteria of tsutsugamushi disease. The specific nested PCR amplification was carried out in 10 cases of tsutsugamushi disease, among which 6 cases amplified the target gene fragment. The positive rate was 60%. The DNA sequence and gene bank sequence of the gene fragment of Origamushi tsutsugamushi virus in Zhuhai were compared. It was found that it had the highest homology with the Korean strain Yonchon strain, which was more than 95%. The phylogenetic tree showed that it was in the same branch as the Karp strain. Conclusion Zhuhai is the natural foci of tsutsugamushi disease, and the genotype of Orientella tsutsugamushi is the most similar to that of Yonchon strain.
【作者单位】：遵义医学院第五附属(珠海)医院感染科;
【基金】：珠海市卫生局课题(No.2011077)~~
【分类号】：R513.2

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