庐江县新型布尼亚病毒及人粒细胞无形体疫源地调查

发布时间：2018-10-05 15:51

【摘要】：目的通过人群、家畜发热伴血小板减少综合征布尼亚病毒(severe fever with thrombocytopenia syndrome bunyavirus,SFTSV)及人粒细胞无形体病(human granulocytic anaplasmosis,HGA)感染状况调查,阐明庐江县是否存在SFTSV和HGA自然疫源地,为科学防治工作提供依据。方法收集分析2009-2013年SFTS和HGA病例流行病学资料。在全县选取1个有病例的镇,采用多阶段随机抽样方法 ,随机抽取病例附近2个村庄,采集自然人群血清100份,15只家畜血块和血清各1份。对血清标本采用双抗体夹心法检测SFTSV抗体和间接荧光法检测人粒细胞无形体病抗体免疫球蛋白G,对动物标本采用巢式聚合酶链反应(polymerase chain reaction,PCR)检测人粒细胞无形体16SrRNA基因和荧光定量反转录PCR检测SFTSV核酸。结果 2009-2013年,共报告9例SFTS和1例HGA,以中老年为主,女性多于男性,夏秋季为发病高峰。人HGA阳性率24%,SFTSV抗体阳性率0%。15份动物标本SFTSV抗体阳性6份,HGA 16SrRNA基因阳性9份,双阳性5份,SFTSV核酸和人粒细胞无形体抗体均阴性。结论该县农村地区存在SFTSV和HGA疫源地,人群和家畜中均有感染,家畜可能是该类疾病的宿主动物。需加强宣传、培训和快速诊断能力。
[Abstract]:Objective to investigate the infection status of human fever associated with thrombocytopenia syndrome (severe fever with thrombocytopenia syndrome bunyavirus,SFTSV) and human granulocytic disease (human granulocytic anaplasmosis,HGA) in Lujiang County, and to clarify whether there are natural foci of SFTSV and HGA in Lujiang County. To provide the basis for scientific prevention and control work. Methods Epidemiological data of SFTS and HGA cases from 2009 to 2013 were collected and analyzed. A town with a case was selected in the whole county. Two villages near the case were randomly selected by multi-stage random sampling method. 100 sera of 15 domestic animals and 1 blood clot of 15 domestic animals were collected from natural population. SFTSV antibody and immunoglobulin G of human granulocytic disease antibody were detected by double antibody sandwich method and indirect fluorescence method respectively. Nested polymerase chain reaction (polymerase chain reaction,PCR) was used to detect human granulocytic 16SrRNA in animal samples. Gene and fluorescence quantitative reverse transcription PCR were used to detect SFTSV nucleic acid. Results from 2009 to 2013, 9 cases of SFTS and 1 case of HGA, were reported mainly in middle and old age, more in female than in male, and the peak was in summer and autumn. The positive rate of SFTSV antibody in 15 animal specimens was 0. 15. 6 of them were positive for SFTSV 16SrRNA gene, 9 were positive for HGA 16SrRNA gene, 5 were positive for SFTSV nucleic acid and human granulocyte anisomorphic antibody. Conclusion there are SFTSV and HGA foci in rural areas of the county, and both human and domestic animals are infected. Livestock may be the host animals of this disease. Communication, training and rapid diagnosis skills need to be strengthened.
【作者单位】：安徽医科大学公共卫生学院流行病与卫生统计学系;庐江县疾病预防控制中心应急办;安徽省疾病预防控制中心;
【分类号】：R512.8

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