全自动酶免方法检测登革病毒抗原抗体的有效性评价

发布时间：2018-10-10 19:46

【摘要】：目的评价全自动酶联免疫吸附(ELISA)实验方法在献血人群中检测登革病毒NS1抗原和Ig M、Ig G抗体的有效性。方法以疾病预防控制中心常规使用的登革病毒NS1抗原和Ig M、Ig G抗体的实验诊断方法作为参照,对全自动ELISA方法的检测结果进行评价。结果全自动ELISA方法检测NS1的灵敏度、特异度、约登指数、阳性预测值、阴性预测值、符合率和Kappa值分别为96.11%、94.81%、0.91、92.51%、97.34%、95.33%和0.903,检测Ig M的灵敏度、特异度、约登指数、阳性预测值、阴性预测值、符合率和Kappa值分别为97.04%、91.11%、0.88、91.61%、96.85%、94.07%和0.881,检测Ig G的灵敏度、特异度、约登指数、阳性预测值、阴性预测值、符合率和Kappa值分别为92.96%、90.00%、0.83、90.29%、92.75%、91.48%和0.830。结论全自动ELISA检测方法检测结果可靠,适用于大规模献血人群的登革病毒感染筛查。
[Abstract]:Objective to evaluate the effectiveness of automatic enzyme-linked immunosorbent assay (ELISA) for detection of dengue virus NS1 antigen and Ig McG antibody in blood donors. Methods the diagnostic methods of NS1 antigen of dengue virus and IgG antibody of Ig were used in the center of disease prevention and control as the reference. The results of automatic ELISA method were evaluated. Results the sensitivity, specificity, Yorden index, positive predictive value, negative predictive value, coincidence rate and Kappa value of the automatic ELISA method for detecting NS1 were 96.111.94.81and 0.903, respectively. The sensitivity, specificity, Yorden index, positive predictive value of Ig M were 95.33% and 0.903, respectively. The negative predictive value, coincidence rate and Kappa value were 97.04 / 91.11 and 0.888 / 91.61and 94.07% and 0.881respectively. The sensitivity, specificity, Yorden index, positive predictive value, negative predictive value, coincidence rate and Kappa value of Ig G were 92.9690.000.830.290.299.75757.48% and 0.830%, respectively. Conclusion the results of automatic ELISA detection are reliable and suitable for screening for dengue virus infection in large blood donors.
【作者单位】：南宁市疾病预防控制中心;南宁中心血站;
【基金】：广西自然科学基金资助项目(基金号2015GXNSFAA139206)
【分类号】：R446.6;R512.8

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