荚膜组织胞浆菌病诊断及与结核病鉴别诊断方法的实验研究

发布时间：2018-10-11 17:56

【摘要】：目的探讨荚膜组织胞浆菌病(HP)诊断及其与结核病鉴别诊断的方法。方法提取荚膜组织胞浆菌素纯蛋白衍生物(P-HTPM),以P-HTPM、荚膜组织胞浆菌素国外参考品和结核菌素纯蛋白衍生物(TB-PPD)为研究试剂,以荚膜组织胞浆菌和结核分枝杆菌免疫的豚鼠和小鼠及其血清为实验对象,采用皮肤试验、酶联免疫吸附试验(ELISA)、胶体金免疫层析试验(GICA)3种方法进行HP诊断及与结核病鉴别诊断的研究。结果成功提取P-HTPM,蛋白纯度为85.8%;P-HTPM与国外参考品的皮肤反应结果一致(P0.05),1 mg/L的P-HTPM与国外参考品等效,P-HTPM与TB-PPD对致敏豚鼠皮肤试验无交叉反应;包被P-HTPM的ELISA检测小鼠抗荚膜组织胞浆菌血清全为阳性,检测抗结核分枝杆菌血清和正常小鼠血清结果均为阴性(P0.01);以P-HTPM抗原为检测线的GICA试纸条检测小鼠抗荚膜组织胞浆菌血清灵敏度为100%(14/14),特异度为93.8%(15/16)。结论以P-HTPM为抗原的动物皮肤试验、ELISA和GICA对HP的诊断及其与结核病的鉴别诊断有重要价值。
[Abstract]:Objective to investigate the (HP) diagnosis of histoplasmosis perfringens and its differential diagnosis with tuberculosis. Methods the purified protein derivatives of histiocystin perfringens (P-HTPM) were extracted. P-HTPM, foreign reference material of histocytoplasmic capsule and pure protein derivative of tuberculin (TB-PPD) were used as research reagents. The guinea pigs and mice immunized with histiocytoplasma perfringens and Mycobacterium tuberculosis and their serum were used for skin test. Enzyme linked immunosorbent assay (Elisa) (ELISA), colloidal gold immunochromatographic assay (GICA) was used to diagnose HP and differentiate it from tuberculosis. Results the protein purity was 85.8%. The results of skin reaction of P-HTPM were consistent with those of foreign reference (P0.05). The P-HTPM of 1 mg/L was equivalent to that of foreign reference. There was no cross reaction between P-HTPM and TB-PPD in skin test of sensitized guinea pigs. The ELISA coated with P-HTPM was all positive for the detection of anti-histoplasms perfringens in mice. The results of anti-Mycobacterium tuberculosis serum and normal mouse serum were negative (P0.01), and the sensitivity and specificity of GICA test strip with P-HTPM antigen were 100% (14 / 14) and 93.8% (15 / 16) respectively. Conclusion the animal skin test with P-HTPM as antigen, ELISA and GICA have important value in the diagnosis of HP and its differential diagnosis with tuberculosis.
【作者单位】：济南市中心医院呼吸内科;重庆医科大学附属第一医院;中国食品药品检定研究院菌苗室;
【基金】：国家“863”项目(2004AA2Z3452)
【分类号】：R519.8;R52

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